Synaptic enrichment and dynamic regulation of the two opposing dopamine receptors within the same neurons

Shun Hiramatsu; Kokoro Saito; Shu Kondo; Hidetaka Katow; Nobuhiro Yamagata; Chun-Fang Wu; Hiromu Tanimoto

doi:10.7554/eLife.98358

Back

Synaptic enrichment and dynamic regulation of the two opposing dopamine receptors within the same neurons

Journal article

Open access

Peer reviewed

Synaptic enrichment and dynamic regulation of the two opposing dopamine receptors within the same neurons

Shun Hiramatsu, Kokoro Saito, Shu Kondo, Hidetaka Katow, Nobuhiro Yamagata, Chun-Fang Wu and Hiromu Tanimoto

eLife, Vol.13, RP98358

01/30/2025

DOI: 10.7554/eLife.98358

PMCID: PMC11781798

PMID: 39882849

Files and links (1)

url

https://doi.org/10.7554/eLife.98358View

Published (Version of record) Open Access

Abstract

Dopamine can play opposing physiological roles depending on the receptor subtype. In the fruit fly , and encode the D - and D -like receptors, respectively, and are reported to oppositely regulate intracellular cAMP levels. Here, we profiled the expression and subcellular localization of endogenous Dop1R1 and Dop2R in specific cell types in the mushroom body circuit. For cell-type-specific visualization of endogenous proteins, we employed reconstitution of split-GFP tagged to the receptor proteins. We detected dopamine receptors at both presynaptic and postsynaptic sites in multiple cell types. Quantitative analysis revealed enrichment of both receptors at the presynaptic sites, with Dop2R showing a greater degree of localization than Dop1R1. The presynaptic localization of Dop1R1 and Dop2R in dopamine neurons suggests dual feedback regulation as autoreceptors. Furthermore, we discovered a starvation-dependent, bidirectional modulation of the presynaptic receptor expression in the protocerebral anterior medial (PAM) and posterior lateral 1 (PPL1) clusters, two distinct subsets of dopamine neurons, suggesting their roles in regulating appetitive behaviors. Our results highlight the significance of the co-expression of the two opposing dopamine receptors in the spatial and conditional regulation of dopamine responses in neurons.

Animals

Dopaminergic Neurons - metabolism

Drosophila melanogaster - genetics

Drosophila melanogaster - metabolism

Drosophila Proteins - genetics

Drosophila Proteins - metabolism

Gene Expression Regulation

Mushroom Bodies - metabolism

Receptors, Dopamine - genetics

Receptors, Dopamine - metabolism

Receptors, Dopamine D1 - genetics

Receptors, Dopamine D1 - metabolism

Receptors, Dopamine D2 - genetics

Receptors, Dopamine D2 - metabolism

Synapses - metabolism

Details

Title: Subtitle: Synaptic enrichment and dynamic regulation of the two opposing dopamine receptors within the same neurons
Creators: Shun Hiramatsu - Tohoku University
Kokoro Saito - Tohoku University
Shu Kondo - Tokyo University of Science
Hidetaka Katow - New York University
Nobuhiro Yamagata - Akita University
Chun-Fang Wu - University of Iowa
Hiromu Tanimoto - Tohoku University
Resource Type: Journal article
Publication Details: eLife, Vol.13, RP98358
DOI: 10.7554/eLife.98358
PMID: 39882849
PMCID: PMC11781798
NLM abbreviation: Elife
ISSN: 2050-084X
eISSN: 2050-084X
Grant note: 20H00519 / Japan Society for the Promotion of Science 22H04829 / Japan Society for the Promotion of Science 17H04765 / Japan Society for the Promotion of Science 20H03246 / Japan Society for the Promotion of Science 17H01378 / Japan Society for the Promotion of Science 19KK0383 / Japan Society for the Promotion of Science JPMJSP2114 / Support for Pioneering Research Initiated by the Next Generation 22H05481 / Japan Society for the Promotion of Science 22KK0106 / Japan Society for the Promotion of Science
Language: English
Date published: 01/30/2025
Academic Unit: Iowa Neuroscience Institute; Biology
Record Identifier: 9984781278702771

Metrics

11 Record Views

See more details