Journal article
Synaptotagmin-7 controls the size of the reserve and resting pools of synaptic vesicles in hippocampal neurons
Cell calcium (Edinburgh), Vol.74, pp.53-60
09/2018
DOI: 10.1016/j.ceca.2018.06.004
PMID: 29957297
Abstract
[Display omitted]
•Synaptotagmins (Syt) are the major calcium sensors in synaptic transmission.•Many steps are calcium dependent in synaptic transmission.•Syt–7 contributes with Syt-1 in triggering vesicle fusion.•The lack of Syt-7 tends to accumulate vesicles in all pools, being significant in the reserve and resting pools.
Continuous neurotransmitter release is subjected to synaptic vesicle availability, which in turn depends on vesicle recycling and the traffic of vesicles between pools. We studied the role of Synaptotagmin-7 (Syt-7) in synaptic vesicle accessibility for release in hippocampal neurons in culture. Synaptic boutons from Syt-7 knockout (KO) mice displayed normal basal secretion with no alteration in the RRP size or the probability of release. However, stronger stimuli revealed an increase in the size of the reserve and resting vesicle pools in Syt-7 KO boutons compared with WT. These data suggest that Syt-7 plays a significant role in the vesicle pool homeostasis and, consequently, in the availability of vesicles for synaptic transmission during strong stimulation, probably, by facilitating advancing synaptic vesicles to the readily releasable pool.
Details
- Title: Subtitle
- Synaptotagmin-7 controls the size of the reserve and resting pools of synaptic vesicles in hippocampal neurons
- Creators
- Elisa Durán - Dpto. Fisiología Médica y Biofísica, Facultad de Medicina, Universidad de Sevilla, 41009 Sevilla, SpainMaría Ángeles Montes - Dpto. Fisiología Médica y Biofísica, Facultad de Medicina, Universidad de Sevilla, 41009 Sevilla, SpainImane Jemal - Dpto. Fisiología Médica y Biofísica, Facultad de Medicina, Universidad de Sevilla, 41009 Sevilla, SpainRachel Satterfield - Research Group Molecular Mechanisms of Synaptic Function, Max Planck Florida Institute, Jupiter, FL 33458. USASamuel Young - Research Group Molecular Mechanisms of Synaptic Function, Max Planck Florida Institute, Jupiter, FL 33458. USAGuillermo Álvarez de Toledo - Dpto. Fisiología Médica y Biofísica, Facultad de Medicina, Universidad de Sevilla, 41009 Sevilla, Spain
- Resource Type
- Journal article
- Publication Details
- Cell calcium (Edinburgh), Vol.74, pp.53-60
- Publisher
- Elsevier Ltd
- DOI
- 10.1016/j.ceca.2018.06.004
- PMID
- 29957297
- ISSN
- 0143-4160
- eISSN
- 1532-1991
- Language
- English
- Date published
- 09/2018
- Academic Unit
- Anatomy and Cell Biology; Iowa Neuroscience Institute; Otolaryngology
- Record Identifier
- 9984025409302771
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