Journal article
Synchronized infection of cell cultures by magnetically controlled virus
Journal of virology, Vol.79(1), pp.622-625
01/2005
DOI: 10.1128/JVI.79.1.622-625.2005
PMCID: PMC538731
PMID: 15596857
Abstract
To override the diffusion-limited adsorption step of viral infection, we magnetically synchronized cell attachment. Human immunodeficiency virus type 1-based lentivirus preparations were rendered magnetically reactive by association with magnetite nanoparticles, 50 nm in diameter. Application of a magnetic field resulted in immediate redistribution of the viral inoculum to the cell-associated state and completion of the productive adsorption process within 1 min. Independent of adsorption time, viral concentration, and diffusion rate, infection subsequently progressed by the receptor-mediated entry mechanism. Synchronization of this rate-limiting step of infection may now be applied to analyze isolated events in the viral replication sequence.
Details
- Title: Subtitle
- Synchronized infection of cell cultures by magnetically controlled virus
- Creators
- Hillel Haim - Department of Virology, Hadassah Medical School, The Hebrew University, Jerusalem, Israel. hhaim@md.huji.ac.ilIsrael SteinerAmos Panet
- Resource Type
- Journal article
- Publication Details
- Journal of virology, Vol.79(1), pp.622-625
- DOI
- 10.1128/JVI.79.1.622-625.2005
- PMID
- 15596857
- PMCID
- PMC538731
- NLM abbreviation
- J Virol
- ISSN
- 0022-538X
- eISSN
- 1098-5514
- Language
- English
- Date published
- 01/2005
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984083887702771
Metrics
30 Record Views