Synectin/syndecan-4 regulate coronary arteriolar growth during development

Eduard I Dedkov; Mathew T Thomas; Milan Sonka; Fuxing Yang; Thomas W Chittenden; John M Rhodes; Michael Simons; Erik L Ritman; Robert J Tomanek

doi:10.1002/dvdy.21201

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Synectin/syndecan-4 regulate coronary arteriolar growth during development

Journal article

Open access

Peer reviewed

Synectin/syndecan-4 regulate coronary arteriolar growth during development

Eduard I Dedkov, Mathew T Thomas, Milan Sonka, Fuxing Yang, Thomas W Chittenden, John M Rhodes, Michael Simons, Erik L Ritman and Robert J Tomanek

Developmental dynamics, Vol.236(7), pp.2004-2010

07/2007

DOI: 10.1002/dvdy.21201

PMID: 17576142

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Abstract

Syndecan-4 and its cytoplasmic binding partner, synectin, are known to play a role in FGF-2 signaling and vascular growth. To determine their roles in coronary artery/arteriolar formation and growth, we compared syndecan-4 and synectin null mice with their wild-type counterparts. Image analysis of arterioles visualized by smooth muscle alpha-actin immunostaining revealed that synectin (-/-) mice had lower arteriolar length and volume densities than wild-type mice. As shown by electron microscopic analysis, arterioles from the two did not differ in morphology, including their endothelial cell junctions, and the organization and distribution of smooth muscle. Using micro-computer tomography, we found that the size and branching patterns of coronary arteries (diameters > 50 microm) were similar for the two groups, a finding that indicates that the growth of arteries is not influenced by a loss of synectin. Syndecan-4 null male mice also had lower arteriolar length densities than their gender wild-type controls. However, female syndecan-4 null mice were characterized by higher arteriolar length and volume densities than their gender-matched wild-type controls. Thus, we conclude that both synectin and syndecan-4 play a role in arteriolar development, a finding that is consistent with previous evidence that FGF-2 plays a role in coronary arterial growth. Moreover, our data reveal that gender influences the arteriolar growth response to syndecan-4 but not to synectin.

Carrier Proteins - physiology

Arterioles - embryology

Syndecan-4 - genetics

Coronary Vessels - embryology

Male

Neuropeptides - physiology

Coronary Vessels - metabolism

Mice, Knockout

Syndecan-4 - physiology

Carrier Proteins - genetics

Adaptor Proteins, Signal Transducing

Animals

Arterioles - metabolism

Female

Mice

Neuropeptides - genetics

Details

Title: Subtitle: Synectin/syndecan-4 regulate coronary arteriolar growth during development
Creators: Eduard I Dedkov - Department of Anatomy and Cell Biology, Carver College of Medicine, The University of Iowa, Iowa City, Iowa 52242, USA
Mathew T Thomas
Milan Sonka
Fuxing Yang
Thomas W Chittenden
John M Rhodes
Michael Simons
Erik L Ritman
Robert J Tomanek
Resource Type: Journal article
Publication Details: Developmental dynamics, Vol.236(7), pp.2004-2010
DOI: 10.1002/dvdy.21201
PMID: 17576142
NLM abbreviation: Dev Dyn
ISSN: 1058-8388
eISSN: 1097-0177
Publisher: United States
Grant note: HL62289 / NHLBI NIH HHS HL53793 / NHLBI NIH HHS R01 HL075446 / NHLBI NIH HHS EB000305 / NIBIB NIH HHS
Language: English
Date published: 07/2007
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Electrical and Computer Engineering; Anatomy and Cell Biology; Radiation Oncology; Injury Prevention Research Center; Ophthalmology and Visual Sciences
Record Identifier: 9984047896202771

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