Journal article
Synergistic effects of antimicrobial components of the human-derived composite amnion-chorion membrane on bacterial growth
Frontiers in cellular and infection microbiology, Vol.14, p.1472737
10/07/2024
DOI: 10.3389/fcimb.2024.1472737
PMCID: PMC11491435
PMID: 39435187
Abstract
The human-derived amnion-chorion membrane (ACM) has endogenous antimicrobial properties, which are important for preventing the colonization and survival of oral bacteria on exposed membranes. This project aimed to decipher the underlying mechanism by identifying the components of ACM that confer antibacterial properties. In addition, the antimicrobial efficacy of these identified components on oral bacteria was assessed.
Four antimicrobial proteins, histone H2A/H2B, cathelicidin LL-37, lactoferrin, and lysozyme, were identified via mass spectrometry in ACM. These proteins were then assessed for their efficacy in killing
Challis. Log-phased bacterial cells were cultured with the commercially available proteins that were identified in ACM, either individually or in combination, at different concentrations. After incubation for 8 or 24 hours, the bacteria were stained with a live/dead viability kit and analyzed via confocal microscopy.
The combination of these proteins effectively killed
in a dose-dependent fashion after 8 or 24 hours of incubation. When each protein was tested individually, it killed
at a much lower efficacy relative to the combinations. The synergistic effects of the antimicrobial protein combinations were also observed in both the viable cell count recovery and minimum inhibitory concentration assays.
By shedding light on the mechanisms in the ACM's antimicrobial property, this study may raise more awareness of the potential benefit of utilization of a membrane with endogenous antimicrobial properties in regeneration surgeries.
Details
- Title: Subtitle
- Synergistic effects of antimicrobial components of the human-derived composite amnion-chorion membrane on bacterial growth
- Creators
- Alexandra Su Brummerhop - The University of Texas Health Science Center at HoustonChun-Teh Lee - The University of Texas Health Science Center at HoustonRobin Weltman - The University of Texas Health Science Center at HoustonGena D Tribble - The University of Texas Health Science Center at HoustonRansome van der Hoeven - University of IowaYulun Chiu - The University of Texas MD Anderson Cancer CenterJianming Hong - The University of Texas Health Science Center at HoustonBing-Yan Wang - The University of Texas Health Science Center at Houston
- Resource Type
- Journal article
- Publication Details
- Frontiers in cellular and infection microbiology, Vol.14, p.1472737
- DOI
- 10.3389/fcimb.2024.1472737
- PMID
- 39435187
- PMCID
- PMC11491435
- NLM abbreviation
- Front Cell Infect Microbiol
- ISSN
- 2235-2988
- eISSN
- 2235-2988
- Publisher
- FRONTIERS MEDIA SA; LAUSANNE
- Grant note
- National Institutes of Health (NIH) High-End Instrumentation Program: 1S10OD012304-01
The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. The UT-M.D. Anderson Proteomics Facility would like to thank the M.D. Anderson Cancer Center and the National Institutes of Health (NIH) High-End Instrumentation Program grant 1S10OD012304-01 for generous support.
- Language
- English
- Date published
- 10/07/2024
- Academic Unit
- Dental Research; Periodontics
- Record Identifier
- 9984737966502771
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