Journal article
Syntaxin 16 regulates lumen formation during epithelial morphogenesis
PloS one, Vol.8(4), pp.e61857-e61857
2013
DOI: 10.1371/journal.pone.0061857
PMCID: PMC3633931
PMID: 23626741
Abstract
The formation and maintenance of cell-cell junctions, both under physiological and pathological conditions, requires the targeting and trafficking of junctional proteins. Proteins of the syntaxin (Stx)-family localize to a variety of subcellular membranes and contribute to intracellular transport of cargo by regulating vesicle fusion events at these sites. Unlike plasma membrane localized Stxs, the roles of endosome- and Golgi-localized stx proteins in epithelial morphogenesis are less understood. Here we show that Stx16- an endosome- and Golgi-localized target-membrane soluble N-ethylmaleimide attachment protein receptor (t-SNARE) that plays a role in membrane trafficking between these compartments - is essential for lumen development. In cultured Madin Darby Canine Kidney (MDCK) cells, Stx16 was selectively upregulated as sparsely plated cells attained confluency. Stx16-depleted confluent monolayers consistently showed lower transepithelial resistance than control monolayers, and failed to maintain endogenous and ectopically expressed E-cadherin at the adherens junctions due to decreased recycling. We further found that whereas cysts formed by MDCK cells cultured in Matrigel have a single hollow lumen, those formed by stx16-depleted counterparts had multiple lumens, due to abnormal orientiation of the mitotic spindle. Finally, a similar role for stx16 function in vivo is indicated by our analysis of pronephric-duct development in zebrafish expressing the claudinB:lynGFP transgene; lack of stx16 function in this structure (in stx16-morphant embryos) led to the development of enlarged, torturous pronephric ducts with more than one lumen. Taken together, our in vitro and in vivo studies establish a role for Stx16 in maintaining the integrity of cell-cell junctions, and thereby in morphogenesis of the kidney epithelial lumen.
Details
- Title: Subtitle
- Syntaxin 16 regulates lumen formation during epithelial morphogenesis
- Creators
- Jae-Joon Jung - Department of Anatomy and Cell Biology, University of Iowa, Iowa City, Iowa, United States of AmericaShivangi M InamdarAjit TiwariDing YeFang LinAmit Choudhury
- Resource Type
- Journal article
- Publication Details
- PloS one, Vol.8(4), pp.e61857-e61857
- DOI
- 10.1371/journal.pone.0061857
- PMID
- 23626741
- PMCID
- PMC3633931
- NLM abbreviation
- PLoS One
- ISSN
- 1932-6203
- eISSN
- 1932-6203
- Publisher
- Public Library of Science; United States
- Grant note
- R01 HL089599 / NHLBI NIH HHS R01HL089599 / NHLBI NIH HHS
- Language
- English
- Date published
- 2013
- Academic Unit
- Anatomy and Cell Biology; Craniofacial Anomalies Research Center
- Record Identifier
- 9984025571002771
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