Journal article
Syntaxin 7 Is Localized to Late Endosome Compartments, Associates with Vamp 8, and Is Required for Late Endosome–Lysosome Fusion
Molecular biology of the cell, Vol.11(9), pp.3137-3153
09/2000
DOI: 10.1091/mbc.11.9.3137
PMCID: PMC14981
PMID: 10982406
Abstract
Protein traffic from the cell surface or thetrans-Golgi network reaches the lysosome via a series of endosomal compartments. One of the last steps in the endocytic pathway is the fusion of late endosomes with lysosomes. This process has been reconstituted in vitro and has been shown to require NSF, α and γ SNAP, and a Rab GTPase based on inhibition by Rab GDI. InSaccharomyces cerevisiae, fusion events to the lysosome-like vacuole are mediated by the syntaxin protein Vam3p, which is localized to the vacuolar membrane. In an effort to identify the molecular machinery that controls fusion events to the lysosome, we searched for mammalian homologues of Vam3p. One such candidate is syntaxin 7. Here we show that syntaxin 7 is concentrated in late endosomes and lysosomes. Coimmunoprecipitation experiments show that syntaxin 7 is associated with the endosomal v-SNARE Vamp 8, which partially colocalizes with syntaxin 7. Importantly, we show that syntaxin 7 is specifically required for the fusion of late endosomes with lysosomes in vitro, resulting in a hybrid organelle. Together, these data identify a SNARE complex that functions in the late endocytic system of animal cells.
Details
- Title: Subtitle
- Syntaxin 7 Is Localized to Late Endosome Compartments, Associates with Vamp 8, and Is Required for Late Endosome–Lysosome Fusion
- Creators
- Barbara M. Mullock - University of CambridgeChez W. Smith - University of IowaGudrun Ihrke - University of CambridgeNicholas A. Bright - University of CambridgeMargaret Lindsay - University of QueenslandEmma J. Parkinson - Women's and Children's HospitalDoug A. Brooks - Women's and Children's HospitalRobert G. Parton - University of QueenslandDavid E. James - University of QueenslandJ. Paul Luzio - University of CambridgeRobert C. Piper - University of Iowa
- Contributors
- Hugh R.B. Pelham (Editor)
- Resource Type
- Journal article
- Publication Details
- Molecular biology of the cell, Vol.11(9), pp.3137-3153
- DOI
- 10.1091/mbc.11.9.3137
- PMID
- 10982406
- PMCID
- PMC14981
- ISSN
- 1059-1524
- eISSN
- 1939-4586
- Language
- English
- Date published
- 09/2000
- Academic Unit
- Molecular Physiology and Biophysics; Medicine Administration; Internal Medicine
- Record Identifier
- 9984297496702771
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