Journal article
Synthesis and Incorporation of Quaternary Ammonium Silane Antimicrobial into Self-Crosslinked Type I Collagen Scaffold: A Hybrid Formulation for 3D Printing
Macromolecular bioscience, Vol.22(3), pp.e2100326-n/a
03/2022
DOI: 10.1002/mabi.202100326
PMID: 34870895
Abstract
Novel 3D-biomaterial scaffold is constructed having a combination of a new quaternary ammonium silane (k21) antimicrobial impregnated in 3D collagen printed scaffolds cross linked with Riboflavin in presence of d-alpha-tocopheryl poly(ethyleneglycol)-1000-succinate. Groups of "0.1% and 0.2% k21", and "0.1% and 0.2% Chlorhexidine (CHX)" are prepared. k21/CHX with neutralized collagen is printed with BioX. Riboflavin is photo-activated and examined using epifluorescence for Aggregatibacter actinomycetemcomitans (7-days). Collagen is examined using TEM and measured for porosity, and shape-fitting. Raman and tandem mass/solid-state are performed with molecular-docking and circular-dichroism. X-ray diffractions, rheological tests, contact angle, and ninhydrin assay are conducted. k21 samples demonstrated collagen aggregates while 0.1% CHX and 0.2% CHX showed irregularities. Porosity of control and "0.1% and 0.2% k21" scaffolds show no differences. Low contact angle, improved elastic-modulus, rigidity, and smaller strain in k21 groups are seen. Bacteria are reduced and strong organic intensities are seen in k21 scaffolds. Simulation shows hydrophobicity/electrostatic interaction. Crosslinking is observed in 0.2% CHX/79% and 0.2% k21/80%. Circular dichroism for k21 are suggestive of triple helix. XRD patterns appear at d = 5.97, 3.03, 2.78, 2.1, and 2.90 A°. 3D-printing of collagen impregnated with quaternary ammonium silane produces a promising scaffold with antimicrobial potency and structural stability.
Details
- Title: Subtitle
- Synthesis and Incorporation of Quaternary Ammonium Silane Antimicrobial into Self-Crosslinked Type I Collagen Scaffold: A Hybrid Formulation for 3D Printing
- Creators
- Ranjeet Ajit Bapat - International Medical UniversitySenthil Kumar Muthusamy - National University of SingaporePreena Sidhu - International Medical UniversityKit-Kay Mak - University of Kuala LumpurAbhishek Parolia - International Medical UniversityMallikarjuna Rao Pichika - University of Kuala LumpurLiang Lin Seow - University of Kuala LumpurCao Tong - National University of SingaporeUmer Daood - International Medical University
- Resource Type
- Journal article
- Publication Details
- Macromolecular bioscience, Vol.22(3), pp.e2100326-n/a
- DOI
- 10.1002/mabi.202100326
- PMID
- 34870895
- ISSN
- 1616-5187
- eISSN
- 1616-5195
- Language
- English
- Date published
- 03/2022
- Academic Unit
- Endodontics
- Record Identifier
- 9984446401902771
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