Journal article
Synthesis and photophysical properties of a fluorescent TREN-type ligand incorporating the coumarin chromophore and its zinc complex
Tetrahedron letters, Vol.46(24), pp.4193-4196
2005
DOI: 10.1016/j.tetlet.2005.04.059
Abstract
A new, UV-excited, fluorescent Zn
2+ indicator was synthesized and the spectral profile of its free and Zn
2+ bound forms was studied. The fluorescent properties of this probe are due to the 7-amino-4-methylcoumarin fluorophore, which is conjugated with the tris(2-aminoethyl)amine (TREN) that functions as the zinc-chelating moiety.
A new, UV-excited fluorescent Zn
2+ indicator was synthesized and the spectral profile of its free and Zn
2+ bound forms were studied. The fluorescent properties of this probe are due to the 7-amino-4-methylcoumarin fluorophore, which is conjugated with the tris(2-aminoethyl)amine (TREN) that functions as the zinc-chelating moiety. The compound exhibits a Zn
2+ dissociation constant of 18.0
μM. The fluorescence spectra of the probe showed a clear shift in excitation wavelength maxima upon Zn
2+ binding, indicating its potential use as ratiometric Zn
2+ indicator.
Details
- Title: Subtitle
- Synthesis and photophysical properties of a fluorescent TREN-type ligand incorporating the coumarin chromophore and its zinc complex
- Creators
- Marianna Dakanali - Division of Organic Chemistry, Department of Chemistry, University of Crete, Heraklion 71 409, Crete, GreeceEmmanuel Roussakis - Division of Organic Chemistry, Department of Chemistry, University of Crete, Heraklion 71 409, Crete, GreeceAlan R Kay - Department of Biological Sciences, 138 BB, University of Iowa, Iowa City, IA 52242, USAHaralambos E Katerinopoulos - Division of Organic Chemistry, Department of Chemistry, University of Crete, Heraklion 71 409, Crete, Greece
- Resource Type
- Journal article
- Publication Details
- Tetrahedron letters, Vol.46(24), pp.4193-4196
- Publisher
- Elsevier Ltd
- DOI
- 10.1016/j.tetlet.2005.04.059
- ISSN
- 0040-4039
- eISSN
- 1873-3581
- Grant note
- DOI: 10.13039/100000065, name: National Institute of Neurological Disorders and Stroke; DOI: 10.13039/501100006408, name: Ministry of National Education and Religious Affairs
- Language
- English
- Date published
- 2005
- Academic Unit
- Biology; Iowa Neuroscience Institute
- Record Identifier
- 9984068355802771
Metrics
10 Record Views