Journal article
Synthesis of homogenous disulfide cross-linked polypeptides by iterative reducible ligation
Biopolymers, Vol.98(6), pp.510-517
2012
DOI: 10.1002/bip.22117
PMID: 23203756
Abstract
A new method of directed solution phase synthesis of polypeptides linked through iterative formation of disulfide bonds is reported. Four dodecapeptides were successfully ligated into a single 48 amino acid polypeptide using an N-terminal Fmoc-thiazolidine and a novel acidic silver trifluoromethanesulfonate thiazolidine hydrolysis to achieve efficient ligation in the presence of internal disulfide bonds. The approach allows the synthesis of homogeneous disulfide cross-linked polypeptides that have application in gene delivery by undergoing a reductively triggered release of DNA.
Details
- Title: Subtitle
- Synthesis of homogenous disulfide cross-linked polypeptides by iterative reducible ligation
- Creators
- Mark D Ericson - Division of Medicinal and Natural Products Chemistry, College of Pharmacy, University of Iowa, IA 52242, USAKevin G Rice
- Resource Type
- Journal article
- Publication Details
- Biopolymers, Vol.98(6), pp.510-517
- DOI
- 10.1002/bip.22117
- PMID
- 23203756
- NLM abbreviation
- Biopolymers
- ISSN
- 0006-3525
- eISSN
- 1097-0282
- Publisher
- United States
- Grant note
- GM087653 / NIGMS NIH HHS T32 GM008365 / NIGMS NIH HHS
- Language
- English
- Date published
- 2012
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Medicinal and Natural Products Chemistry
- Record Identifier
- 9984065695802771
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