Journal article
Synthesis of immunogenic hepatitis A virus particles by recombinant baculoviruses
Vaccine, Vol.11(7), pp.706-712
1993
DOI: 10.1016/0264-410X(93)90253-T
PMID: 8393604
Abstract
Recombinant baculoviruses were constructed which contained the hepatitis A virus (HAV) open reading frame (ORF) under the control of the polyhedrin promoter. Northern blot analysis with an HAV-specific oligonucleotide probe demonstrated a single transcript large enough to include the HAV ORF in
Spodoptera frugiperda cells infected with these recombinants. Immunoblots revealed a 220 kDa protein representing the HAV polyprotein. In addition, proteins which co-migrated with HAV capsid proteins, and several proteins of intermediate size were present, consistent with processing intermediates. HAV antigen was present in cells infected with the recombinant baculoviruses when assessed by solid-phase radioimmunoassay. This HAV antigen had a buoyant density in caesium chloride gradients similar to HAV empty capsids, and elicited HAV neutralizing antibodies in mice.
Details
- Title: Subtitle
- Synthesis of immunogenic hepatitis A virus particles by recombinant baculoviruses
- Creators
- Elliot Rosen - American Biogenetic Sciences, Inc., Reyniers Building, University of Notre Dame, Notre Dame, IN 46556, USAJack T Stapleton - Departments of Internal Medicine, Iowa City VA Medical Center and the University of Iowa College of Medicine, Iowa City, IA 52242, USAJames McLinden - American Biogenetic Sciences, Inc., Reyniers Building, University of Notre Dame, Notre Dame, IN 46556, USA
- Resource Type
- Journal article
- Publication Details
- Vaccine, Vol.11(7), pp.706-712
- DOI
- 10.1016/0264-410X(93)90253-T
- PMID
- 8393604
- NLM abbreviation
- Vaccine
- ISSN
- 0264-410X
- eISSN
- 1873-2518
- Publisher
- Elsevier Ltd
- Language
- English
- Date published
- 1993
- Academic Unit
- Microbiology and Immunology; Infectious Diseases; Internal Medicine
- Record Identifier
- 9984094644802771
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