Journal article
Synthesis of sulfhydryl cross-linking poly(ethylene glycol)-peptides and glycopeptides as carriers for gene delivery
Bioconjugate chemistry, Vol.13(2), pp.232-239
03/2002
DOI: 10.1021/bc010070a
PMID: 11906260
Abstract
Sulfhydryl cross-linking poly(ethylene glycol) (PEG)-peptides and glycopeptides were prepared and tested for spontaneous polymerization by disulfide bond formation when bound to plasmid DNA, resulting in stable PEG-peptide and glycopeptide DNA condensates. A 20 amino acid synthetic peptide possessing a single sulfhydryl group on the N-terminal cysteine, with two or five internal acetamidomethyl (Acm)-protected cysteine residues, was reacted with either PEG vinyl sulfone or iodoacetamide tyrosinamide triantennary N-glycan. Following RP-HPLC purification, Acm groups were removed by silver tetrafluoroborate to generate sulfhydryl cross-linking PEG-peptides and glycopeptide that were characterized by either (1)H NMR or LC-MS. Sulfhydryl cross-linking PEG-peptides and glycopeptides were found to bind to plasmid DNA and undergo disulfide cross-linking resulting in stable DNA condensates with potential utility for in vivo gene delivery.
Details
- Title: Subtitle
- Synthesis of sulfhydryl cross-linking poly(ethylene glycol)-peptides and glycopeptides as carriers for gene delivery
- Creators
- Youmie Park - College of Pharmacy, 428 Church Street, University of Michigan, Ann Arbor, Michigan 48109-1065, USAKai Y KwokChawki BoukarimKevin G Rice
- Resource Type
- Journal article
- Publication Details
- Bioconjugate chemistry, Vol.13(2), pp.232-239
- DOI
- 10.1021/bc010070a
- PMID
- 11906260
- NLM abbreviation
- Bioconjug Chem
- ISSN
- 1043-1802
- eISSN
- 1520-4812
- Publisher
- United States
- Grant note
- GM48049 / NIGMS NIH HHS
- Language
- English
- Date published
- 03/2002
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Medicinal and Natural Products Chemistry
- Record Identifier
- 9984065324402771
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