Journal article
Systematic Analysis of the Protein Interaction Network for the Human Transcription Machinery Reveals the Identity of the 7SK Capping Enzyme
Molecular cell, Vol.27(2), pp.262-274
2007
DOI: 10.1016/j.molcel.2007.06.027
PMCID: PMC4498903
PMID: 17643375
Abstract
We have performed a survey of soluble human protein complexes containing components of the transcription and RNA processing machineries using protein affinity purification coupled to mass spectrometry. Thirty-two tagged polypeptides yielded a network of 805 high-confidence interactions. Remarkably, the network is significantly enriched in proteins that regulate the formation of protein complexes, including a number of previously uncharacterized proteins for which we have inferred functions. The RNA polymerase II (RNAP II)-associated proteins (RPAPs) are physically and functionally associated with RNAP II, forming an interface between the enzyme and chaperone/scaffolding proteins. BCDIN3 is the 7SK snRNA methylphosphate capping enzyme (MePCE) present in an snRNP complex containing both RNA processing and transcription factors, including the elongation factor P-TEFb. Our results define a high-density protein interaction network for the mammalian transcription machinery and uncover multiple regulatory factors that target the transcription machinery.
Details
- Title: Subtitle
- Systematic Analysis of the Protein Interaction Network for the Human Transcription Machinery Reveals the Identity of the 7SK Capping Enzyme
- Creators
- Célia Jeronimo - Laboratory of Gene Transcription and Proteomics Discovery Platform, Institut de recherches cliniques de Montréal, 110 avenue des Pins Ouest, Montréal, QC H2W 1R7, CanadaDiane Forget - Laboratory of Gene Transcription and Proteomics Discovery Platform, Institut de recherches cliniques de Montréal, 110 avenue des Pins Ouest, Montréal, QC H2W 1R7, CanadaAnnie Bouchard - Laboratory of Gene Transcription and Proteomics Discovery Platform, Institut de recherches cliniques de Montréal, 110 avenue des Pins Ouest, Montréal, QC H2W 1R7, CanadaQintong Li - Biochemistry Department, University of Iowa, Iowa City, IA 52242-1109, USAGordon Chua - Banting and Best Department of Medical Research, University of Toronto, Toronto, ON M5G 1L6, CanadaChristian Poitras - Laboratory of Gene Transcription and Proteomics Discovery Platform, Institut de recherches cliniques de Montréal, 110 avenue des Pins Ouest, Montréal, QC H2W 1R7, CanadaCynthia Thérien - Laboratory of Gene Transcription and Proteomics Discovery Platform, Institut de recherches cliniques de Montréal, 110 avenue des Pins Ouest, Montréal, QC H2W 1R7, CanadaDominique Bergeron - Laboratory of Gene Transcription and Proteomics Discovery Platform, Institut de recherches cliniques de Montréal, 110 avenue des Pins Ouest, Montréal, QC H2W 1R7, CanadaSylvie Bourassa - Centre hospitalier universitaire de Québec, Université Laval, Québec, QC G1V 4G2, CanadaJack Greenblatt - Banting and Best Department of Medical Research, University of Toronto, Toronto, ON M5G 1L6, CanadaBenoit Chabot - Département de microbiologie et infectiologie, Université de Sherbrooke, Sherbrooke, QC J1H 5N4, CanadaGuy G Poirier - Centre hospitalier universitaire de Québec, Université Laval, Québec, QC G1V 4G2, CanadaTimothy R Hughes - Banting and Best Department of Medical Research, University of Toronto, Toronto, ON M5G 1L6, CanadaMathieu Blanchette - McGill Centre for Bioinformatics, McGill University, Montréal, QC H3A 2B4, CanadaDavid H Price - Biochemistry Department, University of Iowa, Iowa City, IA 52242-1109, USABenoit Coulombe - Laboratory of Gene Transcription and Proteomics Discovery Platform, Institut de recherches cliniques de Montréal, 110 avenue des Pins Ouest, Montréal, QC H2W 1R7, Canada
- Resource Type
- Journal article
- Publication Details
- Molecular cell, Vol.27(2), pp.262-274
- DOI
- 10.1016/j.molcel.2007.06.027
- PMID
- 17643375
- PMCID
- PMC4498903
- NLM abbreviation
- Mol Cell
- ISSN
- 1097-2765
- eISSN
- 1097-4164
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 2007
- Academic Unit
- Biochemistry and Molecular Biology
- Record Identifier
- 9984024501002771
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