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Systems genetics reveals ITIH5 as a key mediator of adipocyte–Endothelial crosstalk
Journal article   Open access   Peer reviewed

Systems genetics reveals ITIH5 as a key mediator of adipocyte–Endothelial crosstalk

Mingqi Zhou, Leandro M. Velez, Danica Kwan, Lambda Moses, Casey D. Johnson, Christy M. Nguyen, Lillian Mott, Farah Gamie, Mona Fouladi, Hosung Bae, …
Molecular metabolism (Germany), Vol.108, p.102373
06/01/2026
DOI: 10.1016/j.molmet.2026.102373
PMID: 42025695
url
https://doi.org/10.1016/j.molmet.2026.102373View
Published (Version of record) Open Access

Abstract

Proper adipose tissue homeostasis is essential for systemic metabolic health, and its disruption promotes insulin resistance, inflammation, and cardiometabolic risk. Using unbiased systems genetics analyses in mice and humans identified ITIH5 as a central regulator of adipose homeostasis and whole-body metabolism. Acute administration of recombinant ITIH5 with pan-organ sequencing revealed a local adipose function, suppressing recruitment of circulating immune cells. Consistently, ITIH5 treatment in human endothelial cells reduced leukocyte recruitment. We generated temporally controlled, adipocyte-specific ITIH5 overexpression models in mice, which improved adipose architecture, glucose metabolism under high-fat diet conditions, while consistently reducing left ventricular mass and cardiac output regardless of dietary group. Spatial transcriptomics of adipose tissue showed that elevated ITIH5 signaling to endothelia selectively impairs dendritic cell (DC) and B cell activation pathways. Collectively, these findings identify a mechanism whereby natural genetic variation in an adipocyte-secreted protein modulates endothelial–immune interactions in fat, influencing cardiometabolic homeostasis in a diet-dependent manner. •Multi-tissue network and genetic centrality identify ITIH5 as a regulator linking adipose coordination to systemic homeostasis.•Adipocyte-specific AAV models show ITIH5 enhances adipocyte–endothelial signaling and reduces hypertrophy and glucose intolerance.•Spatial transcriptomics maps adipocyte–endothelial–immune crosstalk, showing ITIH5 suppresses adipose immune activation.•Integrated spatial and physiological data link ITIH5 immune suppression in adipose to protection against cardiac remodeling.
Adipose cell crosstalk Adipose-endothelial signaling ITIH5 Systems genetics

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