Journal article
T Cell Receptor Gene Usage in Desmoglein-3-Specific T Lymphocytes from Patients with Pemphigus Vulgaris
Journal of investigative dermatology, Vol.121(6), pp.1365-1372
12/2003
DOI: 10.1111/j.1523-1747.2003.12601.x
PMID: 14675184
Abstract
Pemphigus vulgaris (PV) is an autoimmune disease mediated by autoantibodies against desmoglein-3 (Dsg3). It has been documented that both humoral and cellular autoimmunity play essential roles in the development of PV. Recently, we identified that T cells from PV patients respond to three antigenic fragments on the ectodomain of Dsg3. These T cells are CD4 α/β cells secreting a Th2-like cytokine profile, and responding of Dsg3 in a restriction to HLA-DRBI*0402 or 1401 alleles. Other characteristics of these cells, such as detailed epitope(s) and T cell receptors (TCRs) usage, however, have not been investigated. The purpose of this study is to determine detailed T cell epitope(s) and TCR genes utilized by Dsg3-specific T cells. Here, we found that Dsg3(AA145-192)-specific cells preferentially utilize the TCRVβ13 gene, while Dsg3(AA240-303)- and Dsg3 (AA570-614)-specific cells utilize Vβ7 and Vβ17 genes, respectively. Analysis of TCRVα gene expression, it appears that Vα22 gene is expressed by Dsg3(AA145-192)-specific cells, whereas the Vα10 gene is predominantly utilized by Dsg3(AA240-303)-specific T cells. There are no specific utilization of Vα gene in the group of cells proliferate to Dsg3 (AA570-614). We believe that this information will further our understanding of the properties of autoimmune T cells in patients with PV.
Details
- Title: Subtitle
- T Cell Receptor Gene Usage in Desmoglein-3-Specific T Lymphocytes from Patients with Pemphigus Vulgaris
- Creators
- Mary K Hacker-foegen - Department of Dermatology, Medical College of Wisconsin, Milwaukee, WisconsinJanet A Fairley - Department of Dermatology, Medical College of Wisconsin, Milwaukee, WisconsinMong-Shang Lin - Department of Dermatology, Medical College of Wisconsin, Milwaukee, Wisconsin
- Resource Type
- Journal article
- Publication Details
- Journal of investigative dermatology, Vol.121(6), pp.1365-1372
- Publisher
- Elsevier Inc
- DOI
- 10.1111/j.1523-1747.2003.12601.x
- PMID
- 14675184
- ISSN
- 0022-202X
- eISSN
- 1523-1747
- Language
- English
- Date published
- 12/2003
- Academic Unit
- Dermatology
- Record Identifier
- 9984025430602771
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