Journal article
T Cell-Specific Deficiency in BBSome Component BBS1 Interferes with Selective Immune Responses
American journal of physiology. Regulatory, integrative and comparative physiology, Vol.324(2), pp.R161-R170
02/2023
DOI: 10.1152/ajpregu.00243.2022
PMCID: PMC9844976
PMID: 36534590
Abstract
Bardet Biedl syndrome (BBS) is a genetic condition associated with various clinical features including cutaneous disorders and certain autoimmune and inflammatory diseases pointing to a potential role of BBS proteins in the regulation of immune function. BBS1 protein which is a key component of the BBSome, a protein complex involved in the regulation of cilia function and other cellular processes, has been implicated in the immune synapse assembly by promoting the centrosome polarization to the antigen presenting cells. Here, we assessed the effect of disrupting the BBSome, through Bbs1 gene deletion, in T cells. Interestingly, mice lacking the Bbs1 gene specifically in T cells (T-BBS1
) displayed normal body weight, adiposity, and glucose handling, but have smaller spleens. However, T-BBS1
mice had no change in the proportion and absolute number of B cells and T cells in the spleen and lymph nodes. There was also no alteration in the CD4/CD8 lineage commitment or survival in the thymus of T-BBS1
mice. On the other hand, T-BBS1
mice treated with Imiquimod dermally exhibited significantly higher percentage of CD3-positive splenocytes that was due to CD4 but not CD8 T cell predominance. Notably, we found that T-BBS1
mice had significantly decreased wound closure, an effect that was more pronounced in males indicating that the BBSome plays an important role in T cell-mediated skin repair. Together, these findings implicate the BBSome in the regulation of selective functions of T cells.
Details
- Title: Subtitle
- T Cell-Specific Deficiency in BBSome Component BBS1 Interferes with Selective Immune Responses
- Creators
- Madeliene Stump - Department of Dermatology, University of Iowa Carver College of Medicine, Iowa City, Iowa, United StatesDeng-Fu Guo - Veterans Affairs Health Care System, Iowa City, Iowa, United StatesKamal Rahmouni - University of Iowa
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Regulatory, integrative and comparative physiology, Vol.324(2), pp.R161-R170
- DOI
- 10.1152/ajpregu.00243.2022
- PMID
- 36534590
- PMCID
- PMC9844976
- ISSN
- 0363-6119
- eISSN
- 1522-1490
- Grant note
- IK6 BX006040 / U.S. Department of Veterans Affairs (VA) I01 BX004249 / U.S. Department of Veterans Affairs (VA) T32AI007260 / HHS | National Institutes of Health (NIH) HL084207 / HHS | National Institutes of Health (NIH)
- Language
- English
- Electronic publication date
- 12/19/2022
- Date published
- 02/2023
- Academic Unit
- Iowa Neuroscience Institute; Fraternal Order of Eagles Diabetes Research Center; Neuroscience and Pharmacology; Internal Medicine
- Record Identifier
- 9984339359602771
Metrics
12 Record Views