T Lymphocytes from a Subset of Patients with Pemphigus Vulgaris Respond to Both Desmoglein-3 and Desmoglein-1

Mong-Shang Lin; Sue J Swartz; Argelia Lopez; Xiang Ding; Janet A Fairley; Luis A Diaz

doi:10.1111/1523-1747.ep12340738

Back

T Lymphocytes from a Subset of Patients with Pemphigus Vulgaris Respond to Both Desmoglein-3 and Desmoglein-1

Journal article

Open access

Peer reviewed

T Lymphocytes from a Subset of Patients with Pemphigus Vulgaris Respond to Both Desmoglein-3 and Desmoglein-1

Mong-Shang Lin, Sue J Swartz, Argelia Lopez, Xiang Ding, Janet A Fairley and Luis A Diaz

Journal of investigative dermatology, Vol.109(6), pp.734-737

12/1997

DOI: 10.1111/1523-1747.ep12340738

PMID: 9406813

Files and links (1)

url

https://doi.org/10.1111/1523-1747.ep12340738View

Published (Version of record) Open Access

Abstract

Pemphigus vulgaris and pemphigus foliaceus are cutaneous autoimmune diseases characterized by intraepithelial blisters and autoantibodies to desmosomal glycoproteins. The antigens recognized by pemphigus vulgaris and pemphigus foliaceus autoantibodies are desmoglein- 3 (Dsg3) and desmoglein-1 (Dsg1), respectively. Dsg3 and Dsg1 are members of the desmoglein subfamily of the cadherin supergene family of cell adhesion molecules. It has been well documented that a subset of pemphigus vulgaris sera have IgG reactivity to both Dsg1 and Dsg3, suggesting that Dsg1 may also participate in the autoimmune response of these patients. The cellular mechanisms of T cell autoimmunity in these patients, however, are completely unknown. In this study, we tested the proliferative responses of T lymphocytes from eight pemphigus vulgaris patients after incubation with Dsg3 and Dsg1 fusion proteins. The sera of four of these PV patients showed reactivity with both Dsg1 and Dsg3, whereas the remaining four reacted only with Dsg3. We found that T cells obtained from those patients that exhibited the combined Dsg1/Dsg3 autoantibody reactivity showed a proliferative response after exposure to either Dsg1 or Dsg3 fusion proteins. The cellular responses to both of these recombinant proteins were highly specific and restricted to the CD4-positive T cell population. T cells from pemphigus vulgaris patients with no anti-Dsg1 serum reactivity showed a proliferative response to Dsg3, but not to Dsg1. The Dsg1 fusion protein used in this study has minimal sequence homology with Dsg3. Thus, this study provides the first evidence that T cells from a subset of pemphigus vulgaris patients respond to both Dsg1 and Dsg3.

bullous diseases

desmosome

autoimmunity

autoantibodies

Details

Title: Subtitle: T Lymphocytes from a Subset of Patients with Pemphigus Vulgaris Respond to Both Desmoglein-3 and Desmoglein-1
Creators: Mong-Shang Lin - Department of Dermatology, Medical College of Wisconsin, Wisconsin, U.S.A
Sue J Swartz - Department of Dermatology, Medical College of Wisconsin, Wisconsin, U.S.A
Argelia Lopez - Department of Dermatology, Medical College of Wisconsin, Wisconsin, U.S.A
Xiang Ding - Department of Dermatology, Medical College of Wisconsin, Wisconsin, U.S.A
Janet A Fairley - Department of Dermatology, Medical College of Wisconsin, Wisconsin, U.S.A
Luis A Diaz - Department of Dermatology, Medical College of Wisconsin, Wisconsin, U.S.A
Resource Type: Journal article
Publication Details: Journal of investigative dermatology, Vol.109(6), pp.734-737
Publisher: Elsevier Inc
DOI: 10.1111/1523-1747.ep12340738
PMID: 9406813
ISSN: 0022-202X
eISSN: 1523-1747
Language: English
Date published: 12/1997
Academic Unit: Dermatology
Record Identifier: 9984025668102771

Metrics

15 Record Views

28 Times Cited - Web of Science