Journal article
T and B cell recovery in arthritis adoptively transferred to SCID mice: antigen-specific activation is required for restoration of autopathogenic CD4+ Th1 cells in a syngeneic system
The Journal of immunology (1950), Vol.168(12), pp.6013-6021
06/15/2002
DOI: 10.4049/jimmunol.168.12.6013
PMID: 12055209
Abstract
T cell homeostasis is a physiological function of the immune system that maintains a balance in the numbers and ratios of T cells at the periphery. A self-MHC/self-peptide ligand can induce weak (covert) signals via the TCR, thus providing an extended lifespan for naive T cells. A similar mechanism is responsible for the restoration of immune homeostasis in severe lymphopenic conditions such as those following irradiation or chemotherapy, or upon transfer of lymphocytes to nu/nu or SCID mice. To date, the genetic backgrounds of donor and recipient SCID mice were unmatched in all autoimmune arthritis transfer experiments, and the recovery of lymphoid cells in the host has not been followed. In this study, we present the adoptive transfer of proteoglycan (PG)-induced arthritis using unseparated and T or B cell-depleted lymphocytes from arthritic BALB/c donors to genetically matched syngeneic SCID recipient mice. We demonstrate that selectively recovered lymphoid subsets determine the clinical and immunological status of the recipient. We found that when T cells were depleted (>98% depleted), B cells did not produce PG-specific anti-mouse (auto) Abs unless SCID mice received a second Ag (PG) injection, which promoted the recovery of Ag-specific CD4(+) Th1 cells. Reciprocally, as a result of B cell recovery, high levels of serum anti-PG Abs were found in SCID mice that received B cell-depleted (>99% depleted) T lymphocytes. Our results indicate a selective and highly effective cooperation between CD4(+) T cells and B lymphocytes that is required for the restoration of pathological homeostasis and development of autoimmune arthritis in SCID mice.
Details
- Title: Subtitle
- T and B cell recovery in arthritis adoptively transferred to SCID mice: antigen-specific activation is required for restoration of autopathogenic CD4+ Th1 cells in a syngeneic system
- Creators
- Tamás Bárdos - Section of Molecular Medicine, Departments of Orthopedic Surgery and Biochemistry, Section of Rheumatology, Department of Medicine, and Department of Immunology/Microbiology, Rush-Presbyterian-St. Luke's Medical Center, Rush University, Chicago, IL 60612Katalin MikeczAlison FinneganJian ZhangTibor T Glant
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.168(12), pp.6013-6021
- DOI
- 10.4049/jimmunol.168.12.6013
- PMID
- 12055209
- NLM abbreviation
- J Immunol
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Publisher
- United States
- Grant note
- AR47657 / NIAMS NIH HHS AR45652 / NIAMS NIH HHS AR47412 / NIAMS NIH HHS AR40310 / NIAMS NIH HHS
- Language
- English
- Date published
- 06/15/2002
- Academic Unit
- Pathology
- Record Identifier
- 9984047791302771
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