Journal article
T cell substance P receptor governs antigen-elicited IFN-γ production
American journal of physiology: Gastrointestinal and liver physiology, Vol.284(2), pp.G197-G204
02/01/2003
DOI: 10.1152/ajpgi.00271.2002
PMID: 12388184
Abstract
Substance P (SP) enhances antigen-dependent T cell IFN-γ production. It was determined if a T cell neurokinin-1 receptor (NK-1R) was critical for IFN-γ regulation. T cells from schistosome-infected mice were mixed with splenocytes from uninfected NK-1R knockout (KO) animals. Thus only the schistosome egg antigen-specific T cells expressed NK-1R. The cells were cultured 18 h with or without SP. SP enhanced antigen-induced IFN-γ production fourfold without affecting IL-4 or IL-5 secretion. NK-1R inhibitor blocked this stimulation. Neither purified T cells nor naı̈ve KO splenocytes cultured alone responded to antigen. To further define the importance of T cell NK-1R, we developed a T cell-selective NK-1R KO mouse by reconstituting T cell-deficient Rag mice with NK-1R KO T cells. These mice challanged with schistosomiasis developed abnormal liver granulomas. Granuloma size was smaller in T cell-selective NK-1R KO mice compared with granulomas in Rag reconstituted with normal T cells. Splenocytes and granuloma cells from NK-1R KO mice made less IFN-γ. The mice also made less IgG2a. Thus T cell NK-1R is important for IFN-γ regulation.
Details
- Title: Subtitle
- T cell substance P receptor governs antigen-elicited IFN-γ production
- Creators
- Arthur M Blum - Division of Gastroenterology-Hepatology, Department of Internal Medicine, University of Iowa, Iowa City, Iowa 52242Ahmed Metwali - Division of Gastroenterology-Hepatology, Department of Internal Medicine, University of Iowa, Iowa City, Iowa 52242David E Elliott - Division of Gastroenterology-Hepatology, Department of Internal Medicine, University of Iowa, Iowa City, Iowa 52242Joel V Weinstock - Division of Gastroenterology-Hepatology, Department of Internal Medicine, University of Iowa, Iowa City, Iowa 52242
- Resource Type
- Journal article
- Publication Details
- American journal of physiology: Gastrointestinal and liver physiology, Vol.284(2), pp.G197-G204
- DOI
- 10.1152/ajpgi.00271.2002
- PMID
- 12388184
- ISSN
- 0193-1857
- eISSN
- 1522-1547
- Language
- English
- Date published
- 02/01/2003
- Academic Unit
- Gastroenterology and Hepatology; Internal Medicine
- Record Identifier
- 9984094572002771
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