T cell syncytia induced by HIV release. T cell chemoattractants: demonstration with a newly developed single cell chemotaxis chamber

Damon C Shutt; Leslie M Jenkins; Edward J Carolan; Jack Stapleton; Karla J Daniels; Ronald C Kennedy; David R Soll

doi:10.1242/jcs.111.1.99

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T cell syncytia induced by HIV release. T cell chemoattractants: demonstration with a newly developed single cell chemotaxis chamber

Journal article

Peer reviewed

T cell syncytia induced by HIV release. T cell chemoattractants: demonstration with a newly developed single cell chemotaxis chamber

Damon C Shutt, Leslie M Jenkins, Edward J Carolan, Jack Stapleton, Karla J Daniels, Ronald C Kennedy and David R Soll

Journal of cell science, Vol.111 (1), pp.99-109

01/1998

DOI: 10.1242/jcs.111.1.99

PMID: 9394016

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Abstract

A chemotaxis chamber has been developed to analyze both the velocity and the directionality of individual T cells in gradients of high molecular mass molecules over long periods of time. Employing this chamber, it is demonstrated that syncytia induced by HIV in SUP-T1 cell cultures release two T cell chemoattractants with approximate molecular masses of 30 and 120 kDa. Neither uninfected single cells nor polyethylene glycol-induced syncytia release detectable chemoattractant, suggesting that these chemoattractants are linked to HIV infection. Soluble gp120 functions as a T cell chemoattractant and the addition of anti-gp120 antibody to syncytium-conditioned medium blocks the high molecular mass chemoattractant activity but not the low molecular mass activity. The addition of anti-CD4 antibody to syncytium-conditioned medium also blocks the high molecular mass chemoattractant activity but not the low molecular mass activity. These results demonstrate that HIV-induced T cell syncytia release a low and a high molecular mass T cell chemoattractant, and suggest that the high molecular mass factor is gp120 and that it functions through the CD4 receptor.

Microscopy - methods

CD4 Antigens - immunology

Molecular Weight

Giant Cells - metabolism

Humans

Cell Culture Techniques - methods

HIV Envelope Protein gp120 - metabolism

T-Lymphocytes - virology

Giant Cells - virology

HIV-1 - immunology

Chemotactic Factors - metabolism

T-Lymphocytes - cytology

Microscopy - instrumentation

Chemotaxis - immunology

HIV Envelope Protein gp120 - chemistry

Cell Line, Transformed

Details

Title: Subtitle: T cell syncytia induced by HIV release. T cell chemoattractants: demonstration with a newly developed single cell chemotaxis chamber
Creators: Damon C Shutt - Department of Biological Sciences, University of Iowa, Iowa City, Iowa 52242, USA
Leslie M Jenkins
Edward J Carolan
Jack Stapleton
Karla J Daniels
Ronald C Kennedy
David R Soll
Resource Type: Journal article
Publication Details: Journal of cell science, Vol.111 (1), pp.99-109
DOI: 10.1242/jcs.111.1.99
PMID: 9394016
ISSN: 0021-9533
eISSN: 1477-9137
Grant note: AI40040 / NIAID NIH HHS DE12161 / NIDCR NIH HHS HD18577 / NICHD NIH HHS
Language: English
Date published: 01/1998
Academic Unit: Microbiology and Immunology; Infectious Diseases; Biology; Nursing; Internal Medicine
Record Identifier: 9984094739302771

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