Journal article
T cells undergo rapid ON/OFF but not ON/OFF/ON cycling of cytokine production in response to antigen
The Journal of immunology (1950), Vol.174(2), pp.718-726
01/15/2005
DOI: 10.4049/jimmunol.174.2.718
PMID: 15634891
Abstract
Inflammatory cytokines such as IFN-gamma and TNF produced by Ag-stimulated CD4(+) and CD8(+) T cells are important in defense against microbial infection. However, production of these cytokines must be tightly regulated to prevent immunopathology. Previous studies, conducted with BALB/c mice, have suggested that 1) CD8(+) T cells maintain IFN-gamma production but transiently produce TNF in the continued presence of Ag and 2) lymphocytic choriomeningitis virus-specific and in vitro-propagated effector CD8(+) T cells could rapidly cycle IFN-gamma production ON/OFF/ON in response to Ag exposure, removal, and re-exposure. In contrast with CD8(+) T cells, our results show that Listeria monocytogenes-specific CD4(+) T cells from C57BL/6 mice rapidly initiate (ON cycling) and maintain production of both IFN-gamma and TNF in the continued presence of Ag. Upon Ag removal, production of both cytokines rapidly ceases (OFF cycling). However, if the initial stimulation was maximal, Ag-specific CD4(+) T cells were unable to reinitiate cytokine production after a second Ag exposure. Furthermore, L. monocytogenes-specific CD8(+) T cells in the same mice and lymphocytic choriomeningitis virus-specific CD8(+) T cells in BALB/c mice also underwent ON/OFF cycling, but if the initial Ag stimulus was maximal, they could not produce IFN-gamma after Ag re-exposure. As the initial Ag dose was reduced, the number of cells producing cytokine in response to the second Ag exposure exhibited a corresponding increase. However, T cells that were marked for IFN-gamma secretion during the first stimulation did not contribute cytokine production during the second stimulation. Thus, T cells are not able to undergo rapid ON/OFF/ON cytokine cycling in vitro in response to Ag.
Details
- Title: Subtitle
- T cells undergo rapid ON/OFF but not ON/OFF/ON cycling of cytokine production in response to antigen
- Creators
- Gail A Corbin - Department of Microbiology, University of Iowa, Iowa City 52242, USAJohn T Harty
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.174(2), pp.718-726
- DOI
- 10.4049/jimmunol.174.2.718
- PMID
- 15634891
- NLM abbreviation
- J Immunol
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Publisher
- United States
- Grant note
- AI42767 / NIAID NIH HHS AI46653 / NIAID NIH HHS AI50073 / NIAID NIH HHS
- Language
- English
- Date published
- 01/15/2005
- Academic Unit
- Pathology
- Record Identifier
- 9984047871702771
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