Journal article
T regulatory cells participate in the control of germinal centre reactions
Immunology, Vol.133(4), pp.452-468
08/2011
DOI: 10.1111/j.1365-2567.2011.03456.x
PMCID: PMC3143357
PMID: 21635248
Abstract
Germinal centre (GC) reactions are central features of T-cell-driven B-cell responses, and the site where antibody-producing cells and memory B cells are generated. Within GCs, a range of complex cellular and molecular events occur which are critical for the generation of high affinity antibodies. These processes require exquisite regulation not only to ensure the production of desired antibodies, but to minimize unwanted autoreactive or low affinity antibodies. To assess whether T regulatory (Treg) cells participate in the control of GC responses, immunized mice were treated with an anti-glucocorticoid-induced tumour necrosis factor receptor-related protein (GITR) monoclonal antibody (mAb) to disrupt Treg-cell activity. In anti-GITR-treated mice, the GC B-cell pool was significantly larger compared with control-treated animals, with switched GC B cells composing an abnormally high proportion of the response. Dysregulated GCs were also observed regardless of strain, T helper type 1 or 2 polarizing antigens, and were also seen after anti-CD25 mAb treatment. Within the spleens of immunized mice, CXCR5(+) and CCR7(-) Treg cells were documented by flow cytometry and Foxp3(+) cells were found within GCs using immunohistology. Final studies demonstrated administration of either anti-transforming growth factor-β or anti-interleukin-10 receptor blocking mAb to likewise result in dysregulated GCs, suggesting that generation of inducible Treg cells is important in controlling the GC response. Taken together, these findings indicate that Treg cells contribute to the overall size and quality of the humoral response by controlling homeostasis within GCs.
Details
- Title: Subtitle
- T regulatory cells participate in the control of germinal centre reactions
- Creators
- Carla-Maria Alexander - Department of Pathology, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA 52242, USALorraine T TygrettAlexander W BoydenKristy L WolniakKevin L LeggeThomas J Waldschmidt
- Resource Type
- Journal article
- Publication Details
- Immunology, Vol.133(4), pp.452-468
- Publisher
- England
- DOI
- 10.1111/j.1365-2567.2011.03456.x
- PMID
- 21635248
- PMCID
- PMC3143357
- ISSN
- 0019-2805
- eISSN
- 1365-2567
- Grant note
- R01 AA019438 / NIAAA NIH HHS T32 GM007337 / NIGMS NIH HHS R01AA019438 / NIAAA NIH HHS
- Language
- English
- Date published
- 08/2011
- Academic Unit
- Microbiology and Immunology; Pathology
- Record Identifier
- 9984047890902771
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