Journal article
TASBE Flow Analytics: A Package for Calibrated Flow Cytometry Analysis
ACS synthetic biology, Vol.8(7), pp.1524-1529
07/19/2019
DOI: 10.1021/acssynbio.8b00533
PMID: 31053031
Abstract
Flow cytometry is a powerful method for high-throughput precision measurement of cell fluorescence and size. Effective use of this tool for quantification of synthetic biology devices and circuits, however, generally requires careful application of complex multistage workflows for calibration, filtering, and analysis with appropriate statistics. The TASBE Flow Analytics package provides a free, open, and accessible implementation of such workflows in a form designed for high-throughput analysis of large synthetic biology data sets. Given a set of experimental samples and controls, this package can process them to output calibrated data, quantitative analyses and comparisons, automatically generated figures, and detailed debugging and diagnostic reports in both human-readable and machine-readable forms. TASBE Flow Analytics can be used through a simple user-friendly interactive Excel interface, as a library supporting Matlab, Octave, or Python interactive sessions, or as a component integrated into automated workflows.
Details
- Title: Subtitle
- TASBE Flow Analytics: A Package for Calibrated Flow Cytometry Analysis
- Creators
- Jacob Beal - RTX (United States)Cassandra Overney - RTX (United States)Aaron Adler - RTX (United States)Fusun Yaman - RTX (United States)Lisa Tiberio - RTX (United States)Meher Samineni - University of Utah
- Resource Type
- Journal article
- Publication Details
- ACS synthetic biology, Vol.8(7), pp.1524-1529
- DOI
- 10.1021/acssynbio.8b00533
- PMID
- 31053031
- NLM abbreviation
- ACS Synth Biol
- ISSN
- 2161-5063
- eISSN
- 2161-5063
- Language
- English
- Date published
- 07/19/2019
- Academic Unit
- Electrical and Computer Engineering
- Record Identifier
- 9984627237302771
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