Journal article
TBP paralogs accommodate metazoan- and vertebrate-specific developmental gene regulation
The EMBO journal, Vol.26(17), pp.3900-3909
09/05/2007
DOI: 10.1038/sj.emboj.7601822
PMCID: PMC1994123
PMID: 17703192
Abstract
In addition to TATA-binding protein (TBP), a key factor for transcription initiation, the metazoan-specific TBP-like factor TLF/TRF2 and the vertebrate-specific factor TBP2/TRF3 are known to be required for transcription of specific subsets of genes. We have combined an antisense-knockdown approach with transcriptome profiling to determine the significance and biological role of TBP-independent transcription in early gastrula-stage
Xenopus laevis
embryos. Here, we report that, although each of the TBP family members is essential for embryonic development, relatively few genes depend on TBP in the embryo. Most of the transcripts that depend on TBP in the embryo are also expressed maternally and in adult stages, and show no functional specialization. In contrast, TLF is linked to preferential expression in embryos and shows functional specialization in catabolism. A requirement for TBP2 is linked to vertebrate-specific embryonic genes and ventral-specific expression. Therefore TBP paralogs are essential for the gene-regulatory repertoire that is directly linked to early embryogenesis.
Details
- Title: Subtitle
- TBP paralogs accommodate metazoan- and vertebrate-specific developmental gene regulation
- Creators
- Ulrike G Jacobi - Department of Molecular Biology, Faculty of Science, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen, Nijmegen, The NetherlandsRobert C Akkers - Department of Molecular Biology, Faculty of Science, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen, Nijmegen, The NetherlandsElisabeth S Pierson - Department of General Instruments, Radboud University Nijmegen, Nijmegen, The NetherlandsDaniel L Weeks - Department of Biochemistry, University of Iowa, Iowa City, IA, USAJohn M Dagle - Department of Biochemistry, University of Iowa, Iowa City, IA, USAGert Jan C Veenstra - Department of Molecular Biology, Faculty of Science, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen, Nijmegen, The Netherlands
- Resource Type
- Journal article
- Publication Details
- The EMBO journal, Vol.26(17), pp.3900-3909
- Publisher
- Nature Publishing Group
- DOI
- 10.1038/sj.emboj.7601822
- PMID
- 17703192
- PMCID
- PMC1994123
- ISSN
- 0261-4189
- eISSN
- 1460-2075
- Alternative title
- Alternative transcription initiation in development
- Language
- English
- Date published
- 09/05/2007
- Academic Unit
- Stead Family Department of Pediatrics; Epidemiology; Biochemistry and Molecular Biology; Neonatology
- Record Identifier
- 9984025297302771
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