TCR alpha beta chains associate with the plasma membrane independently of CD3 and TCR zeta chains in murine primary T cells

Jian Zhang; Konstantin Salojin; Jian-Xin Gao; Mark Cameron; Carsten Geisler; Terry L Delovitch

doi:10.4049/jimmunol.161.6.2930

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TCR alpha beta chains associate with the plasma membrane independently of CD3 and TCR zeta chains in murine primary T cells

Journal article

Peer reviewed

TCR alpha beta chains associate with the plasma membrane independently of CD3 and TCR zeta chains in murine primary T cells

Jian Zhang, Konstantin Salojin, Jian-Xin Gao, Mark Cameron, Carsten Geisler and Terry L Delovitch

The Journal of immunology (1950), Vol.161(6), pp.2930-2937

09/15/1998

DOI: 10.4049/jimmunol.161.6.2930

PMID: 9743355

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Abstract

The TCR is a multisubunit complex composed of the clonotypic alpha/beta disulfide-linked heterodimer and noncovalently linked invariant CD3 gamma epsilon and CD3 delta epsilon and TCR zeta chains. Recent studies demonstrate that the surface expression of CD3 components can occur independently of the clonotypic TCR complexes in both thymocytes and splenic T cells. In this study, we report that free noncovalently associated TCR alpha beta heterodimers that exist independently of CD3 and TCR zeta chains are expressed on the cell surface of immature thymocytes and peripheral T cells, but not of T cell lines and T cell hybridomas. This suggests that the regulation of surface expression of TCR alpha beta heterodimers differs between primary T cells and T cell lines or T cell hybridomas. The isolation and biochemical characterization of surface clonotype-independent CD3 complexes and free membrane-associated TCR alpha beta complexes may provide a structural basis for the quantitative difference in amount of T cell proliferation stimulated by anti-CD3 epsilon and anti-TCR beta.

T-Lymphocyte Subsets - immunology

Hybridomas - metabolism

Jurkat Cells - metabolism

Humans

Receptors, Antigen, T-Cell, alpha-beta - immunology

Lymphocyte Activation - immunology

Receptors, Antigen, T-Cell, alpha-beta - biosynthesis

Cell Membrane - metabolism

Membrane Proteins - metabolism

T-Lymphocyte Subsets - ultrastructure

Calcium-Binding Proteins - metabolism

Immune Sera - pharmacology

Receptors, Antigen, T-Cell - metabolism

Mice, Inbred C57BL

Calnexin

CD3 Complex - metabolism

Macromolecular Substances

Cell Differentiation - immunology

Animals

Jurkat Cells - immunology

T-Lymphocyte Subsets - metabolism

CD3 Complex - immunology

Hybridomas - immunology

Cell Membrane - immunology

Mice

Mice, Inbred BALB C

Receptors, Antigen, T-Cell, alpha-beta - metabolism

Details

Title: Subtitle: TCR alpha beta chains associate with the plasma membrane independently of CD3 and TCR zeta chains in murine primary T cells
Creators: Jian Zhang - Autoimmunity/Diabetes Group, The John P. Robarts Research Institute, University of Western Ontario, London, Canada
Konstantin Salojin
Jian-Xin Gao
Mark Cameron
Carsten Geisler
Terry L Delovitch
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.161(6), pp.2930-2937
Publisher: United States
DOI: 10.4049/jimmunol.161.6.2930
PMID: 9743355
ISSN: 0022-1767
eISSN: 1550-6606
Language: English
Date published: 09/15/1998
Academic Unit: Pathology
Record Identifier: 9984047855402771

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