TCR-mediated functions are enhanced in activated peripheral blood T cells isolated from leucocyte reduction systems

Mikaela M Tremblay; Jon C D Houtman

doi:10.1016/j.jim.2014.11.009

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TCR-mediated functions are enhanced in activated peripheral blood T cells isolated from leucocyte reduction systems

Journal article

Peer reviewed

TCR-mediated functions are enhanced in activated peripheral blood T cells isolated from leucocyte reduction systems

Mikaela M Tremblay and Jon C D Houtman

Journal of immunological methods, Vol.416, pp.137-145

01/2015

DOI: 10.1016/j.jim.2014.11.009

PMCID: PMC4324009

PMID: 25462023

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Abstract

Buffy coats are the most common method for the acquisition of activated primary human T cells for research or clinical applications, but recently leukocyte reduction system (LRS) cones have emerged as a viable source for these cells. In this study, we determined if activated human T cells derived from buffy coats or LRS cones had different functionality. No changes in the expression of surface receptors were observed except for a significant increase in CD44 expression on T cells isolated from LRS cones. LRS cone-derived T cells trended towards higher receptor-mediated cytokine production and had significantly increased donor-to-donor variability in IFN-γ production. TCR-induced ERK1/ERK2 and AKT phosphorylation was also increased in T cells isolated from LRS cones. In conclusion, LRS cones are an excellent source of T cells for clinical and research applications, but these cells have subtle functional differences from T cells isolated using standard buffy coats.

Leukocytes - immunology

Lymphocyte Activation - immunology

Proto-Oncogene Proteins c-akt - immunology

Humans

Interferon-gamma - immunology

Receptors, Antigen, T-Cell - immunology

T-Lymphocytes - immunology

Hyaluronan Receptors - immunology

Phosphorylation - immunology

MAP Kinase Signaling System - immunology

Details

Title: Subtitle: TCR-mediated functions are enhanced in activated peripheral blood T cells isolated from leucocyte reduction systems
Creators: Mikaela M Tremblay - Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, United States
Jon C D Houtman - Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, United States. Electronic address: jon-houtman@uiowa.edu
Resource Type: Journal article
Publication Details: Journal of immunological methods, Vol.416, pp.137-145
DOI: 10.1016/j.jim.2014.11.009
PMID: 25462023
PMCID: PMC4324009
NLM abbreviation: J Immunol Methods
ISSN: 0022-1759
eISSN: 1872-7905
Publisher: Elsevier BV
Grant note: R01 CA136729 / NCI NIH HHS
Language: English
Date published: 01/2015
Academic Unit: Microbiology and Immunology; Internal Medicine
Record Identifier: 9984094526502771

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