Journal article
TCR usage and functional capabilities of human gamma delta T cells at birth
The Journal of immunology (1950), Vol.153(9), pp.3979-3988
11/01/1994
DOI: 10.4049/jimmunol.153.9.3979
PMID: 7930606
Abstract
Little is known about the gamma delta T cells in human neonatal umbilical cord blood. To compare neonatal cord blood and adult blood gamma delta T cells, we studied the V gamma and V delta gene segment usage in these populations by flow cytometry, and we derived cord blood gamma delta T cell clones to determine their functional capabilities. Unlike adult blood gamma delta T cells that predominantly express V gamma 2V delta 2 TCRs, neonatal cord blood gamma delta T cells expressed diverse V gamma and V delta gene segments paired in a variety of combinations rarely observed in adults. gamma delta T cell clones derived from neonatal cord blood similarly expressed a diverse array of TCRs. These cord blood-derived gamma delta T cell clones had weak cytolytic activity when assayed for K562 tumor cell killing, lectin-mediated cytolysis, and redirected cytolysis. They also expressed lower levels of the CD2, LFA-1, and CD45RO cell surface receptors as compared with strongly cytolytic adult gamma delta T cell clones. These properties of the cord blood-derived gamma delta T cell clones, weak cytotoxic activity and low adhesion molecule expression, were similar to the properties of the CD4+ subset of adult gamma delta T cells. Thus, neonatal gamma delta T cells are functionally different from the majority of adult gamma delta T cells and display a distinct TCR repertoire and accessory molecule profile.
Details
- Title: Subtitle
- TCR usage and functional capabilities of human gamma delta T cells at birth
- Creators
- Craig T Morita - Department of Rheumatology and Immunology, Brigham and Women's Hospital, Boston, MA 02115Christina M ParkerMichael B BrennerHamid Band - Brigham and Women's Hospital
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.153(9), pp.3979-3988
- DOI
- 10.4049/jimmunol.153.9.3979
- PMID
- 7930606
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Grant note
- CA47724 / NCI NIH HHS AI00903 / NIAID NIH HHS GM49342 / NIGMS NIH HHS
- Language
- English
- Date published
- 11/01/1994
- Academic Unit
- Immunology; Internal Medicine
- Record Identifier
- 9984094658102771
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