TCRβ Chain That Forms Peptide-Independent Alloreactive TCR Transfers Reduced Reactivity with Irrelevant Peptide/MHC Complex

Fabio R Santori; Zoran Popmihajlov; Vladimir P Badovinac; Courtney Smith; Sasa Radoja; John T Harty; Stanislav Vukmanović

doi:10.4049/jimmunol.178.10.6109

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TCRβ Chain That Forms Peptide-Independent Alloreactive TCR Transfers Reduced Reactivity with Irrelevant Peptide/MHC Complex

Journal article

Peer reviewed

TCRβ Chain That Forms Peptide-Independent Alloreactive TCR Transfers Reduced Reactivity with Irrelevant Peptide/MHC Complex

Fabio R Santori, Zoran Popmihajlov, Vladimir P Badovinac, Courtney Smith, Sasa Radoja, John T Harty and Stanislav Vukmanović

The Journal of immunology (1950), Vol.178(10), pp.6109-6114

05/15/2007

DOI: 10.4049/jimmunol.178.10.6109

PMID: 17475836

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Abstract

A major feature of the TCR repertoire is strong alloreactivity. Peptides presented by allogeneic MHC are irrelevant for recognition by a subset of alloreactive T cells. To characterize peptide-independent TCRs at the molecular level, we forced the expression of a TCRbeta chain isolated from a peptide-independent alloreactive CD8+ T cell line. The alloreactive TCR repertoire in the transgenic mouse was peptide dependent. However, analysis of essential TCR contacts formed during the recognition of self-MHC-restricted Ag showed that fewer contacts with peptide were established by the transgenic TCRbeta chain, and that this was compensated by additional contacts formed by endogenous TCRalpha chains. Thus, reduced interaction with the peptide appears to be a transferable feature of the peptide-independent TCRbeta chain. In addition, these findings demonstrate that reactivity to peptides is preferred over the reactivity to MHC during the formation of the TCR repertoire.

Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor - physiology

Receptors, Antigen, T-Cell, alpha-beta - administration & dosage

Ovalbumin - metabolism

Lymphocyte Activation - genetics

H-2 Antigens - genetics

Receptors, Antigen, T-Cell, alpha-beta - biosynthesis

CD8-Positive T-Lymphocytes - metabolism

Peptide Fragments - genetics

Peptide Fragments - metabolism

H-2 Antigens - metabolism

Mice, Inbred C57BL

Peptide Fragments - administration & dosage

Antigen Presentation - genetics

Mice, Transgenic

Peptide Fragments - biosynthesis

Receptors, Antigen, T-Cell, alpha-beta - genetics

Mice, Knockout

beta 2-Microglobulin - deficiency

H-2 Antigens - immunology

Animals

Cell Line, Tumor

Mice

Mice, Inbred BALB C

Receptors, Antigen, T-Cell, alpha-beta - metabolism

CD8-Positive T-Lymphocytes - immunology

beta 2-Microglobulin - genetics

Details

Title: Subtitle: TCRβ Chain That Forms Peptide-Independent Alloreactive TCR Transfers Reduced Reactivity with Irrelevant Peptide/MHC Complex
Creators: Fabio R Santori - Michael Heidelberger Division of Immunology, Department of Pathology and New York University Cancer Center, New York University School of Medicine, New York, NY 10016, USA
Zoran Popmihajlov
Vladimir P Badovinac
Courtney Smith
Sasa Radoja
John T Harty
Stanislav Vukmanović
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.178(10), pp.6109-6114
DOI: 10.4049/jimmunol.178.10.6109
PMID: 17475836
NLM abbreviation: J Immunol
ISSN: 0022-1767
eISSN: 1550-6606
Publisher: United States
Grant note: AI48837 / NIAID NIH HHS AI42767 / NIAID NIH HHS AI41573 / NIAID NIH HHS
Language: English
Date published: 05/15/2007
Academic Unit: Microbiology and Immunology; Pathology
Record Identifier: 9984047792002771

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