TERC is not a major gene in human neural tube defects

Lisa P BENZ; Frances E SWIFT; Timothy M GEORGE; Felicia L GRAHAM; David S ENTERLINE; Elizabeth C MELVIN; Preston HAMMOCK; John R GILBERT; Marcy C SPEER; Alexander G BASSUK; John A KESSLER

doi:10.1002/bdra.20057

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TERC is not a major gene in human neural tube defects

Journal article

Peer reviewed

TERC is not a major gene in human neural tube defects

Lisa P BENZ, Frances E SWIFT, Timothy M GEORGE, Felicia L GRAHAM, David S ENTERLINE, Elizabeth C MELVIN, Preston HAMMOCK, John R GILBERT, Marcy C SPEER, Alexander G BASSUK, …

Birth defects research. A Clinical and molecular teratology, Vol.70(8), pp.531-533

2004

DOI: 10.1002/bdra.20057

PMID: 15329831

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Abstract

A method was developed for fast and efficient isolation of DNA from formalin-fixed, paraffin-embedded tissue sections for subsequent use in PCRs and DNA hybridization assays. The method relies on the use of a sonicating water bath to disrupt tissue samples to which a small amount of micro-sized glass beads have been added. The sonicating glass beads provide fast and efficient physical shearing of fixed tissue sections, allowing for quick release and solubilization of the DNA. The extraction process from paraffin section to amplifiable target DNA takes 30 minutes. The method eliminates the need for repetitive solvent extractions and exhaustive proteinase K digestion. PCR amplification of human genomic and viral target sequences was successfully carried out on DNA isolated from a number of different types of normal and infected tissues.

Fundamental and applied biological sciences. Psychology

Biological and medical sciences

Embryology: invertebrates and vertebrates. Teratology

Teratology. Teratogens

Details

Title: Subtitle: TERC is not a major gene in human neural tube defects
Creators: Lisa P BENZ - Center for Human Genetics, Duke University Medical Center, Durham, North Carolina, United States
Frances E SWIFT - Center for Human Genetics, Duke University Medical Center, Durham, North Carolina, United States
Timothy M GEORGE - Department of Surgery, Duke University Medical Center, Durham, North Carolina, United States
Felicia L GRAHAM - Center for Human Genetics, Duke University Medical Center, Durham, North Carolina, United States
David S ENTERLINE - Department of Radiology, Duke University Medical Center, Durham, North Carolina, United States
Elizabeth C MELVIN - Center for Human Genetics, Duke University Medical Center, Durham, North Carolina, United States
Preston HAMMOCK - Center for Human Genetics, Duke University Medical Center, Durham, North Carolina, United States
John R GILBERT - Center for Human Genetics, Duke University Medical Center, Durham, North Carolina, United States
Marcy C SPEER - Center for Human Genetics, Duke University Medical Center, Durham, North Carolina, United States
Alexander G BASSUK - Northwestern University's Feinberg School of Medicine, Chicago Illinois, United States
John A KESSLER - Northwestern University's Feinberg School of Medicine, Chicago Illinois, United States
Resource Type: Journal article
Publication Details: Birth defects research. A Clinical and molecular teratology, Vol.70(8), pp.531-533
DOI: 10.1002/bdra.20057
PMID: 15329831
NLM abbreviation: Birth Defects Res A Clin Mol Teratol
ISSN: 1542-0752
eISSN: 1542-0760
Publisher: Wiley; Hoboken, NJ
Language: English
Date published: 2004
Academic Unit: Neurology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Neurology (Pediatrics)
Record Identifier: 9984020600402771

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