Journal article
TFIIA Plays a Role in the Response to Oxidative Stress
Eukaryotic cell, Vol.5(7), pp.1081-1090
07/2006
DOI: 10.1128/EC.00071-06
PMCID: PMC1489289
PMID: 16835452
Abstract
ABSTRACT
To characterize the role of the general transcription factor TFIIA in the regulation of gene expression by RNA polymerase II, we examined the transcriptional profiles of TFIIA mutants of
Saccharomyces cerevisiae
using DNA microarrays. Whole-genome expression profiles were determined for three different mutants with mutations in the gene coding for the small subunit of TFIIA,
TOA2
. Depending on the particular mutant strain, approximately 11 to 27% of the expressed genes exhibit altered message levels. A search for common motifs in the upstream regions of the pool of genes decreased in all three mutants yielded the binding site for Yap1, the transcription factor that regulates the response to oxidative stress. Consistent with a TFIIA-Yap1 connection, the TFIIA mutants are unable to grow under conditions that require the oxidative stress response. Underexpression of Yap1-regulated genes in the TFIIA mutant strains is not the result of decreased expression of Yap1 protein, since immunoblot analysis indicates similar amounts of Yap1 in the wild-type and mutant strains. In addition, intracellular localization studies indicate that both the wild-type and mutant strains localize Yap1 indistinguishably in response to oxidative stress. As such, the decrease in transcription of Yap1-dependent genes in the TFIIA mutant strains appears to reflect a compromised interaction between Yap1 and TFIIA. This hypothesis is supported by the observations that Yap1 and TFIIA interact both in vivo and in vitro. Taken together, these studies demonstrate a dependence of Yap1 on TFIIA function and highlight a new role for TFIIA in the cellular mechanism of defense against reactive oxygen species.
Details
- Title: Subtitle
- TFIIA Plays a Role in the Response to Oxidative Stress
- Creators
- Susan M. Kraemer - Colorado State UniversityDavid A. Goldstrohm - Colorado State UniversityAnn Berger - PharmaciaSusan Hankey - PharmaciaSherry A. Rovinsky - University of IowaW. Scott Moye‐Rowley - Department of Physiology and Biophysics, University of Iowa, Iowa City, Iowa 52242Laurie A. Stargell - Colorado State University
- Resource Type
- Journal article
- Publication Details
- Eukaryotic cell, Vol.5(7), pp.1081-1090
- DOI
- 10.1128/EC.00071-06
- PMID
- 16835452
- PMCID
- PMC1489289
- NLM abbreviation
- Eukaryot Cell
- ISSN
- 1535-9778
- eISSN
- 1535-9786
- Publisher
- American Society for Microbiology
- Language
- English
- Date published
- 07/2006
- Academic Unit
- Molecular Physiology and Biophysics; Internal Medicine
- Record Identifier
- 9984297601302771
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