TFIID can be rate limiting in vivo for TATA-containing, but not TATA-lacking, RNA polymerase II promoters

John Colgan; James L Manley

doi:10.1101/gad.6.2.304

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TFIID can be rate limiting in vivo for TATA-containing, but not TATA-lacking, RNA polymerase II promoters

Journal article

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TFIID can be rate limiting in vivo for TATA-containing, but not TATA-lacking, RNA polymerase II promoters

John Colgan and James L Manley

Genes & development, Vol.6(2), pp.304-315

1992

DOI: 10.1101/gad.6.2.304

PMID: 1737620

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Abstract

We have studied the effect of exogenous expression of the basal transcription factor TFIID on the activities of several different TATA-containing and TATA-lacking promoters. Overexpression of TFIID from a transfected plasmid in Drosophila Schneider cells resulted in substantial concentration-dependent increases in expression from a cotransfected minimal TATA-containing promoter. Overexpression of TFIID activated expression from all TATA-containing promoters tested, with the maximum level of activation being inversely proportional to the strength of the promoter. In contrast, expression from TATA-less promoters was not enhanced, and could in fact be reduced, by increased expression of TFIID. Consistent with these findings overexpression of TFIID had opposite effects on Sp1-mediated activation observed from minimal synthetic promoters consisting of Sp1-binding sites and either a TATA box or initiator element. We discuss the significance of these results in terms of the role of TFIID in the initiation of transcription and as a possible regulatory target for expression from TATA-containing promoters, as well as the role TFIID may play in expression from TATA-less promoters.

Molecular Genetics

Fundamental and applied biological sciences. Psychology

Biological and medical sciences

Transcription. Transcription factor. Splicing. Rna processing

Molecular and cellular biology

Details

Title: Subtitle: TFIID can be rate limiting in vivo for TATA-containing, but not TATA-lacking, RNA polymerase II promoters
Creators: John Colgan - Columbia univ., dep. biological sci., New York 10027, United States
James L Manley - Columbia univ., dep. biological sci., New York 10027, United States
Resource Type: Journal article
Publication Details: Genes & development, Vol.6(2), pp.304-315
Publisher: Cold Spring Harbor Laboratory; Cold Spring Harbor, NY
DOI: 10.1101/gad.6.2.304
PMID: 1737620
ISSN: 0890-9369
eISSN: 1549-5477
Language: English
Date published: 1992
Academic Unit: Anatomy and Cell Biology; Immunology; Internal Medicine
Record Identifier: 9984025403302771

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