Journal article
TGF-β and IL-10 regulation of IFN-γ produced in Th2-type schistosome granulomas requires IL-12
American journal of physiology: Gastrointestinal and liver physiology, Vol.281(4), pp.G940-G946
10/01/2001
DOI: 10.1152/ajpgi.2001.281.4.G940
PMID: 11557514
Abstract
Interleukin-10 (IL-10) and transforming growth factor-β (TGF-β) regulate CD4+T cell interferon-γ (IFN-γ) secretion in schistosome granulomas. The role of IL-12 was determined using C57BL/6 and CBA mice. C57BL/6 IL-4 −/− granuloma cells were stimulated to produce IFN-γ when cultured with IL-10 or TGF-β neutralizing monoclonal antibody. In comparison, C57BL/6 wild-type (WT) control granuloma cells produced less IFN-γ. IL-12, IL-18, and soluble egg antigen stimulated IFN-γ release from C57BL/6 IL-4 −/− and WT mice. IFN-γ production in C57 IL-4 −/− and WT granulomas was IL-12 dependent, because IL-12 blockade partly abrogated IFN-γ secretion after stimulation. All granuloma cells released IL-12 (p70 and p40), and IL-12 production remained constant after anti-TGF-β, anti-IL-10, recombinant IL-18, or antigen stimulation. C57 WT and IL-4 −/− mouse granuloma cells expressed IL-12 receptor (IL-12R) β1-subunit mRNA but little β2 mRNA. TGF-β or IL-10 blockade did not influence β1 or β2 mRNA expression. CBA mouse dispersed granuloma cells released no measurable IFN-γ, produced IL-12 p70 and little p40, and expressed IL-12R β2 and little β1 mRNA. In T helper 2 (Th2) granulomas of C57BL/6 WT and IL-4 −/− mice, cells produce IL-12 (for IFN-γ production) and IL-10 and TGF-β modulate IFN-γ secretion via mechanisms independent of IL-12 and IL-12R mRNA regulation. We found substantial differences in control of granuloma IFN-γ production and IL-12 circuitry in C57BL/6 and CBA mice.
Details
- Title: Subtitle
- TGF-β and IL-10 regulation of IFN-γ produced in Th2-type schistosome granulomas requires IL-12
- Creators
- Khurram Qadir - Division of Gastroenterology-Hepatology, Department of Internal Medicine, University of Iowa, Iowa City, Iowa 52242Ahmed Metwali - Division of Gastroenterology-Hepatology, Department of Internal Medicine, University of Iowa, Iowa City, Iowa 52242Arthur M Blum - Division of Gastroenterology-Hepatology, Department of Internal Medicine, University of Iowa, Iowa City, Iowa 52242Jie Li - Division of Gastroenterology-Hepatology, Department of Internal Medicine, University of Iowa, Iowa City, Iowa 52242David E Elliott - Division of Gastroenterology-Hepatology, Department of Internal Medicine, University of Iowa, Iowa City, Iowa 52242Joel V Weinstock - Division of Gastroenterology-Hepatology, Department of Internal Medicine, University of Iowa, Iowa City, Iowa 52242
- Resource Type
- Journal article
- Publication Details
- American journal of physiology: Gastrointestinal and liver physiology, Vol.281(4), pp.G940-G946
- DOI
- 10.1152/ajpgi.2001.281.4.G940
- PMID
- 11557514
- NLM abbreviation
- Am J Physiol Gastrointest Liver Physiol
- ISSN
- 0193-1857
- eISSN
- 1522-1547
- Language
- English
- Date published
- 10/01/2001
- Academic Unit
- Gastroenterology and Hepatology; Internal Medicine
- Record Identifier
- 9984094758802771
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