TGFβ2 and TGFβ3 Have Separate and Sequential Activities during Epithelial–Mesenchymal Cell Transformation in the Embryonic Heart

Angelique S Boyer; Ingrid I Ayerinskas; Eric B Vincent; Lisa A McKinney; Daniel L Weeks; Raymond B Runyan

doi:10.1006/dbio.1999.9211

Back

TGFβ2 and TGFβ3 Have Separate and Sequential Activities during Epithelial–Mesenchymal Cell Transformation in the Embryonic Heart

Journal article

Open access

Peer reviewed

TGFβ2 and TGFβ3 Have Separate and Sequential Activities during Epithelial–Mesenchymal Cell Transformation in the Embryonic Heart

Angelique S Boyer, Ingrid I Ayerinskas, Eric B Vincent, Lisa A McKinney, Daniel L Weeks and Raymond B Runyan

Developmental biology, Vol.208(2), pp.530-545

04/15/1999

DOI: 10.1006/dbio.1999.9211

PMID: 10191064

Files and links (1)

url

https://doi.org/10.1006/dbio.1999.9211View

Published (Version of record) Open Access

Abstract

Heart valve formation is initiated by an epithelial-mesenchymal cell transformation (EMT) of endothelial cells in the atrioventricular (AV) canal. Mesenchymal cells formed from cardiac EMTs are the initial cellular components of the cardiac cushions and progenitors of valvular and septal fibroblasts. It has been shown that transforming growth factor beta (TGFbeta) mediates EMT in the AV canal, and TGFbeta1 and 2 isoforms are expressed in the mouse heart while TGFbeta 2 and 3 are expressed in the avian heart. Depletion of TGFbeta3 in avian or TGFbeta2 in mouse leads to developmental defects of heart tissue. These observations raise questions as to whether multiple TGFbeta isoforms participate in valve formation. In this study, we examined the localization and function of TGFbeta2 and TGFbeta3 in the chick heart during EMT. TGFbeta2 was present in both endothelium and myocardium before and after EMT. TGFbeta2 antibody inhibited endothelial cell-cell separation. In contrast, TGFbeta3 was present only in the myocardium before EMT and was in the endothelium at the initiation of EMT. TGFbeta3 antibodies inhibited mesenchymal cell formation and migration into the underlying matrix. Both TGFbeta2 and 3 increased fibrillin 2 expression. However, only TGFbeta2 treatment increased cell surface beta-1,4-galactosyltransferase expression. These data suggest that TGFbeta2 and TGFbeta3 are sequentially and separately involved in the process of EMT. TGFbeta2 mediates initial endothelial cell-cell separation while TGFbeta3 is required for the cell morphological change that enables the migration of cells into the underlying ECM.

Antigens, Differentiation

Transforming Growth Factor beta - immunology

Antibody Specificity

Antisense Elements (Genetics)

Oligonucleotide Probes

Myocardium - chemistry

Epithelial Cells - drug effects

Heart - embryology

Mesoderm - drug effects

Mesoderm - cytology

Chick Embryo

Antibodies - pharmacology

Animals

In Situ Hybridization

Protein Isoforms - metabolism

Cell Differentiation - drug effects

Epithelial Cells - cytology

Protein Isoforms - immunology

Transforming Growth Factor beta - metabolism

Endothelium, Vascular - embryology

Details

Title: Subtitle: TGFβ2 and TGFβ3 Have Separate and Sequential Activities during Epithelial–Mesenchymal Cell Transformation in the Embryonic Heart
Creators: Angelique S Boyer - Department of Cell Biology and Anatomy, University of Arizona, Tucson, Arizona, 85724, USA
Ingrid I Ayerinskas
Eric B Vincent
Lisa A McKinney
Daniel L Weeks
Raymond B Runyan
Resource Type: Journal article
Publication Details: Developmental biology, Vol.208(2), pp.530-545
Publisher: United States
DOI: 10.1006/dbio.1999.9211
PMID: 10191064
ISSN: 0012-1606
eISSN: 1095-564X
Grant note: HL20984 / NHLBI NIH HHS HL42242 / NHLBI NIH HHS
Language: English
Date published: 04/15/1999
Academic Unit: Stead Family Department of Pediatrics; Biochemistry and Molecular Biology
Record Identifier: 9984024416502771

Metrics

48 Record Views

176 Times Cited - Web of Science

See more details