Journal article
THE EFFECT OF IRON ON THE DISTRIBUTION OF SUPEROXIDE AND HYDROXYL RADICALS AS SEEN BY SPIN TRAPPING AND ON THE SUPEROXIDE DISMUTASE ASSAY
Photochemistry and photobiology, Vol.28(4‐5), pp.693-695
10/1978
DOI: 10.1111/j.1751-1097.1978.tb07001.x
PMID: 216030
Abstract
— Using the spin trap 5,5‐dimethyl‐1‐pyrroline‐1‐oxide we have demonstrated the presence of OH in the xanthine‐xanthine oxidase system when iron and/or iron‐EDTA (ethylenediamine‐tetraacetic acid) is present. With increasing iron (or iron‐EDTA) concentration the intensity of the O2‐ spin adduct decreased while that of OH increased. However, use of diethylenetriaminepentaacetic acid (DETAPAC) as a metal chelator in the reaction mixture suppressed the OH spin adduct signal while maintaining the intensity of the signal from the O2‐ spin adduct.
Use of EDTA to eliminate the interfering effects of metal ions in the superoxide dismutase assay employing xanthine oxidase and nitroblue tetrazolium introduces an artifact from the iron present. The interference in the assay from metal ions, including iron, can be eliminated with use of DETAPAC as a metal chelator. Thus, it is possible to make comparisons of measured superoxide dismutase activities even when there are variations in the amount of iron present in the samples.
Details
- Title: Subtitle
- THE EFFECT OF IRON ON THE DISTRIBUTION OF SUPEROXIDE AND HYDROXYL RADICALS AS SEEN BY SPIN TRAPPING AND ON THE SUPEROXIDE DISMUTASE ASSAY
- Creators
- Garry R BuettnerLarry W OberleySusan W. H. Chan Leuthauser
- Resource Type
- Journal article
- Publication Details
- Photochemistry and photobiology, Vol.28(4‐5), pp.693-695
- Publisher
- Blackwell Publishing Ltd; Oxford, UK
- DOI
- 10.1111/j.1751-1097.1978.tb07001.x
- PMID
- 216030
- ISSN
- 0031-8655
- eISSN
- 1751-1097
- Number of pages
- 3
- Language
- English
- Date published
- 10/1978
- Academic Unit
- Radiation Oncology
- Record Identifier
- 9984047619502771
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