Journal article
THE IMMUNOSUPPRESSIVE MECHANISM OF TOTAL LYMPHOID IRRADIATION: I. THE EFFECT ON IL-2 PRODUCTION AND IL-2 RECEPTOR EXPRESSION
Transplantation, Vol.48(3), pp.499-505
09/1989
DOI: 10.1097/00007890-198909000-00029
PMID: 2571207
Abstract
Total lymphoid irradiation is an effective immunosuppressive therapy used to prepare patients for organ transplantation and to treat several autoimmune diseases. We have used the mouse model to investigate the mechanism of TLI-induced immunosuppression. Mice were irradiated with 250-rad daily fractions to a total dose of 3500 rads. Splenocytes from control and TLI-treated mice were analyzed for IL-2 production, IL-2 receptor expression after mitogen stimulation, and percent L3T4 positive cells. At two weeks following the completion of TLI, IL-2 production from whole spleen populations had decreased 90% compared with controls (TLI mean IL-2 units = 25 +/- 9.0, control mean = 277 +/- 104). IL-2 receptor expression on splenocytes following Con A stimulation was decreased 80% compared with controls (TLI mean percentage of IL-2 receptor expressing cells = 16.2 +/- 4.1, control mean = 82.0 +/- 7.5). L3T4+ cells that expressed IL-2 receptor were also decreased following TLI (TLI mean = 4.4 +/- 1.4, control mean = 22.0 +/- 3.8), as were proliferative responses to Con A and PHA. Addition of recombinant mouse IL-2 did not restore IL-2 receptor expression or proliferative responses. Whole TLI-treated splenocytes did not suppress IL-2 production or proliferative responses of normal splenocytes. These immunologic abnormalities recovered over time, and by 8 weeks post TLI IL-2 production, IL-2 receptor expression, L3T4+ cell numbers and proliferative responses had returned toward normal. These results suggest that TLI therapy transiently depletes IL-2 producing, IL-2 receptor expressing, and mitogen responsive lymphocytes. The immunosuppression is not mediated through a suppressor cell and is independent of IL-2 production.
Details
- Title: Subtitle
- THE IMMUNOSUPPRESSIVE MECHANISM OF TOTAL LYMPHOID IRRADIATION: I. THE EFFECT ON IL-2 PRODUCTION AND IL-2 RECEPTOR EXPRESSION
- Creators
- ELIZABETH H FIELDGAIL C BECKER
- Resource Type
- Journal article
- Publication Details
- Transplantation, Vol.48(3), pp.499-505
- DOI
- 10.1097/00007890-198909000-00029
- PMID
- 2571207
- ISSN
- 0041-1337
- eISSN
- 1534-6080
- Language
- English
- Date published
- 09/1989
- Academic Unit
- Immunology; Internal Medicine
- Record Identifier
- 9984094616002771
Metrics
7 Record Views