Journal article
THE ROLE OF INTERLEUKIN 6 IN INTERFERON-γ PRODUCTION IN THERMALLY INJURED MICE
Cytokine (Philadelphia, Pa.), Vol.12(11), pp.1669-1675
11/2000
DOI: 10.1006/cyto.2000.0768
PMID: 11052818
Abstract
Following traumatic injury, patients suffer from compromised immunity increasing their susceptibility to infection. Previous studies from this laboratory demonstrated that female BALB/c mice subjected to a 15% total body surface area (TBSA) scald injury exhibit a decrease in cell-mediated immunity 10 days post-burn. Studies described herein revealed that concanavalin A (Con A; 2μg/ml)-stimulated splenocytes from sham treated animals produced 3557±853pg/ml of IFN-γ while splenocytes from burn injured animals released two-fold more cytokine (P<0.05). To determine whether leukocyte production of IFN-γ was under the influence of macrophages, splenic macrophage supernatants generated from burned animals were incubated with splenic lymphocytes from sham and burn animals. The amount of IFN-γ released by lymphocytes from sham animals increased when cultured with macrophages from burned mice (P<0.05). This suggests that the increase in IFN-γ production by unfractionated splenocytes in burned mice relative to sham treated animals is macrophage-dependent. Macrophage supernatants from burned mice released twice as much IL-6 as supernatants from sham animals (P<0.05), and when IL-6 was blocked in vivo, the amount of IFN-γ production in burned mice decreased to sham levels (P<0.05). Thus, IL-6 mediates IFN-γ production following burn.
Details
- Title: Subtitle
- THE ROLE OF INTERLEUKIN 6 IN INTERFERON-γ PRODUCTION IN THERMALLY INJURED MICE
- Creators
- Elizabeth A Durbin - Department of Cell Biology, Neurobiology, and Anatomy, Loyola University Medical Center, 2160 South First Avenue, Maywood, IL, 60153, USAMeredith S Gregory - Department of Cell Biology, Neurobiology, and Anatomy, Loyola University Medical Center, 2160 South First Avenue, Maywood, IL, 60153, USAKelly A.N Messingham - Department of Cell Biology, Neurobiology, and Anatomy, Loyola University Medical Center, 2160 South First Avenue, Maywood, IL, 60153, USAChristine V Fontanilla - Department of Cell Biology, Neurobiology, and Anatomy, Loyola University Medical Center, 2160 South First Avenue, Maywood, IL, 60153, USALisa A Duffner - Department of Cell Biology, Neurobiology, and Anatomy, Loyola University Medical Center, 2160 South First Avenue, Maywood, IL, 60153, USAElizabeth J Kovacs - Department of Cell Biology, Neurobiology, and Anatomy, Loyola University Medical Center, 2160 South First Avenue, Maywood, IL, 60153, USA
- Resource Type
- Journal article
- Publication Details
- Cytokine (Philadelphia, Pa.), Vol.12(11), pp.1669-1675
- Publisher
- Elsevier Ltd
- DOI
- 10.1006/cyto.2000.0768
- PMID
- 11052818
- ISSN
- 1043-4666
- eISSN
- 1096-0023
- Language
- English
- Date published
- 11/2000
- Academic Unit
- Dermatology
- Record Identifier
- 9984025672402771
Metrics
20 Record Views