Journal article
THE SPIN TRAPPING OF SUPEROXIDE AND HYDROXYL FREE RADICALS WITH DMPO (5,5-DIMETHYLPYRROLINE-N-OXIDE): MORE ABOUT IRON
Free radical research communications, Vol.19(1), pp.S79-S87
1993
DOI: 10.3109/10715769309056s79
PMCID: PMC4669197
PMID: 8282235
Abstract
The reaction of superoxide with the spin trap DMPO (5,5-dimethylpyrroline-N-oxide) is widely used to study superoxide production as well as issues associated with the superoxide-related formation of hydroxyl radical. However, the interpretation of observed intensities of DMPO/•OOH and DMPO/•OH signals in electron paramagnetic resonance spin trapping experiments is not without its difficulties. In this paper, I report experiments that demonstrate:
That the flux of superoxide formation in a DMPO spin trapping experiment can alter the apparent importance of weak DMPO/•OH signals;
That iron can influence the DMPO/•OOH spin trapping results;
That there is very little spontaneous breakdown of DMPO/•OOH to form DMPO/•OH.
Details
- Title: Subtitle
- THE SPIN TRAPPING OF SUPEROXIDE AND HYDROXYL FREE RADICALS WITH DMPO (5,5-DIMETHYLPYRROLINE-N-OXIDE): MORE ABOUT IRON
- Creators
- GARRY R BUETTNER - ESR Facility/EMRB 68, College of Medicine, The University of Iowa, Iowa City, IA 52242 USA
- Resource Type
- Journal article
- Publication Details
- Free radical research communications, Vol.19(1), pp.S79-S87
- DOI
- 10.3109/10715769309056s79
- PMID
- 8282235
- PMCID
- PMC4669197
- NLM abbreviation
- Free Radic Res Commun
- ISSN
- 8755-0199
- eISSN
- 1029-2470
- Language
- English
- Date published
- 1993
- Academic Unit
- Radiation Oncology
- Record Identifier
- 9984047630902771
Metrics
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