Journal article
TNFα Triggers an Augmented Inflammatory Response in Brain Neurons from Dahl Salt-Sensitive Rats Compared with Normal Sprague Dawley Rats
Cellular and molecular neurobiology, Vol.42(6), pp.1787-1800
08/01/2022
DOI: 10.1007/s10571-021-01056-9
PMID: 33625627
Abstract
Tumor Necrosis Factor (TNF)-α is a proinflammatory cytokine (PIC) and has been implicated in a variety of illness including cardiovascular disease. The current study investigated the inflammatory response trigged by TNFα in both cultured brain neurons and the hypothalamic paraventricular nucleus (PVN), a key cardiovascular relevant brain area, of the Sprague Dawley (SD) rats. Our results demonstrated that TNF
α
treatment induces a dose- and time-dependent increase in mRNA expression of PICs including Interleukin (IL)-1β and Interleukin-6 (IL6); chemokines including C–C Motif Chemokine Ligand 5 (CCL5) and C–C Motif Chemokine Ligand 12 (CCL12), inducible nitric oxide synthase (iNOS), as well as transcription factor NF-kB in cultured brain neurons from neonatal SD rats. Consistent with this finding, immunostaining shows that TNFα treatment increases immunoreactivity of IL1β, CCL5, iNOS and stimulates activation or expression of NF-kB, in both cultured brain neurons and the PVN of adult SD rats. We further compared mRNA expression of the aforementioned genes in basal level as well as in response to TNFα challenge between SD rats and Dahl Salt-sensitive (Dahl-S) rats, an animal model of salt-sensitive hypertension. Dahl-S brain neurons presented higher baseline levels as well as greater response to TNFα challenge in mRNA expression of CCL5, iNOS and IL1β. Furthermore, central administration of TNFα caused significant higher response in CCL12 in the PVN of Dahl-S rats. The increased inflammatory response to TNFα in Dahl-S rats may be indicative of an underlying mechanism for enhanced pressor reactivity to salt intake in the Dahl-S rat model.
Details
- Title: Subtitle
- TNFα Triggers an Augmented Inflammatory Response in Brain Neurons from Dahl Salt-Sensitive Rats Compared with Normal Sprague Dawley Rats
- Creators
- Huanjia Gao - Guangzhou University of Chinese MedicineJeremy Bigalke - Michigan Technological UniversityEnshe Jiang - Michigan Technological UniversityYuanyuan Fan - Michigan Technological UniversityBojun Chen - Guangzhou University of Chinese MedicineQing-Hui Chen - Michigan Technological UniversityZhiying Shan - Michigan Technological University
- Resource Type
- Journal article
- Publication Details
- Cellular and molecular neurobiology, Vol.42(6), pp.1787-1800
- DOI
- 10.1007/s10571-021-01056-9
- PMID
- 33625627
- NLM abbreviation
- Cell Mol Neurobiol
- ISSN
- 0272-4340
- eISSN
- 1573-6830
- Publisher
- Springer US
- Number of pages
- 14
- Grant note
- Michigan Tech and Portage Health Foundation Research Excellence Fund NIHR15HL150703 / National Institutes of Health (http://dx.doi.org/10.13039/100000002)
- Language
- English
- Date published
- 08/01/2022
- Academic Unit
- Health, Sport, and Human Physiology
- Record Identifier
- 9985219305302771
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