Tailoring weight ratio of PCL/PLA in electrospun three-dimensional nanofibrous scaffolds and the effect on osteogenic differentiation of stem cells

Tao Xu; Qingqing Yao; Jacob M Miszuk; Hanna J Sanyour; Zhongkui Hong; Hongli Sun; Hao Fong

doi:10.1016/j.colsurfb.2018.07.004

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Tailoring weight ratio of PCL/PLA in electrospun three-dimensional nanofibrous scaffolds and the effect on osteogenic differentiation of stem cells

Journal article

Open access

Peer reviewed

Tailoring weight ratio of PCL/PLA in electrospun three-dimensional nanofibrous scaffolds and the effect on osteogenic differentiation of stem cells

Tao Xu, Qingqing Yao, Jacob M Miszuk, Hanna J Sanyour, Zhongkui Hong, Hongli Sun and Hao Fong

Colloids and surfaces, B, Biointerfaces, Vol.171, pp.31-39

11/01/2018

DOI: 10.1016/j.colsurfb.2018.07.004

PMCID: PMC6174100

PMID: 30005288

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Abstract

Three-dimensional (3D) scaffolds as artificial ECMs have been extensively studied to mimic the critical features of natural ECMs. To develop more clinically relevant 3D scaffolds, electrospun nanofibrous scaffolds with different weight ratios of PCL/PLA (i.e., 100/0, 60/40, and 20/80) were fabricated via the thermally induced (nanofiber) self-agglomeration (TISA) method. The hypothesis was that, with the weight ratio increase of stiffer and more bioactive PLA in the 3D PCL/PLA blend scaffolds, the osteogenic differentiation of human mesenchymal stem cells (hMSCs) would be enhanced. The results indicated that, all of the 3D scaffolds were elastic/resilient and possessed interconnected and hierarchical pores with sizes from sub-microns to ∼300 μm; therefore, the morphological structures of these scaffolds were similar to those of natural ECMs. The PLA80 scaffolds exhibited the best overall properties in terms of density, porosity, water absorption capacity, mechanical properties, bioactivity, and cell viability. Furthermore, with increasing the PLA weight ratio, the alkaline phosphatase (ALP) activity, calcium content, and gene expression level were also increased, probably due to the improved stiffness/bioactivity of scaffold. Hence, the novel 3D electrospun PLA80 nanofibrous scaffold might be desired/favorable for the osteogenic differentiation of hMSCs.

Cell Differentiation - drug effects

Cell Survival - drug effects

Humans

Mesenchymal Stem Cells - cytology

Mesenchymal Stem Cells - drug effects

Nanofibers - chemistry

Osteogenesis - drug effects

Particle Size

Polyesters - chemistry

Polyesters - pharmacology

Surface Properties

Details

Title: Subtitle: Tailoring weight ratio of PCL/PLA in electrospun three-dimensional nanofibrous scaffolds and the effect on osteogenic differentiation of stem cells
Creators: Tao Xu - South Dakota School of Mines and Technology
Qingqing Yao - University of South Dakota
Jacob M Miszuk - University of South Dakota
Hanna J Sanyour - University of South Dakota
Zhongkui Hong - University of South Dakota
Hongli Sun - University of South Dakota
Hao Fong - South Dakota School of Mines and Technology
Resource Type: Journal article
Publication Details: Colloids and surfaces, B, Biointerfaces, Vol.171, pp.31-39
DOI: 10.1016/j.colsurfb.2018.07.004
PMID: 30005288
PMCID: PMC6174100
NLM abbreviation: Colloids Surf B Biointerfaces
ISSN: 0927-7765
eISSN: 1873-4367
Grant note: R03 DE027491 / NIDCR NIH HHS
Language: English
Date published: 11/01/2018
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Craniofacial Anomalies Research Center; Oral and Maxillofacial Surgery; Dental Research
Record Identifier: 9984367620002771

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