Journal article
Targeted Gene Delivery with a Low Molecular Weight Glycopeptide Carrier
Bioconjugate chemistry, Vol.6(3), pp.283-291
05/01/1995
DOI: 10.1021/bc00033a008
PMID: 7632800
Abstract
A low molecular weight glycopeptide carrier was prepared by coupling a tyrosinamide-triantennary oligosaccharide to dp19 poly-L-lysine resulting in a 1:1 conjugate. The glycopeptide carrier complexed with plasmid DNA as evidenced by displacement of intercalated dye, light scattering by condensed DNA, and immobility of complexed DNA upon agarose gel electrophoresis. DNA-carrier complexes were endocytosed into HepG2 cells via the asialoglycoprotein receptor due to recognition of terminal galactose residues on the oligosaccharide. The resulting luciferase reporter gene expression was dramatically influenced by the solubility of complexes, the extent of complexation, and the presence of the lysosomotropic agent chloroquine. The results suggest that low molecular weight glycopeptides may be suitable for further development as well-defined DNA carriers for receptor-mediated gene delivery in vivo.
Details
- Title: Subtitle
- Targeted Gene Delivery with a Low Molecular Weight Glycopeptide Carrier
- Creators
- Manpreet S WadhwaDaren L KnoellAnthony P YoungKevin G Rice
- Resource Type
- Journal article
- Publication Details
- Bioconjugate chemistry, Vol.6(3), pp.283-291
- DOI
- 10.1021/bc00033a008
- PMID
- 7632800
- NLM abbreviation
- Bioconjug Chem
- ISSN
- 1043-1802
- eISSN
- 1520-4812
- Publisher
- American Chemical Society
- Language
- English
- Date published
- 05/01/1995
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Medicinal and Natural Products Chemistry
- Record Identifier
- 9984065697902771
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