Journal article
Targeted, LCMS-based Metabolomics for Quantitative Measurement of NAD+ Metabolites
Computational and Structural Biotechnology Journal, Vol.4(5), e201301012
2013
DOI: 10.5936/csbj.201301012
PMCID: PMC3962138
PMID: 24688693
Abstract
Nicotinamide adenine dinucleotide (NAD + ) is a coenzyme for hydride transfer reactions and a substrate for sirtuins and other NAD + -consuming enzymes. The abundance of NAD + , NAD + biosynthetic intermediates, and related nucleotides reflects the metabolic state of cells and tissues. High performance liquid chromatography (HPLC) followed by ultraviolet-visible (UV-Vis) spectroscopic analysis of NAD + metabolites does not offer the specificity and sensitivity necessary for robust quantification of complex samples. Thus, we developed a targeted, quantitative assay of the NAD + metabolome with the use of HPLC coupled to mass spectrometry. Here we discuss NAD + metabolism as well as the technical challenges required for reliable quantification of the NAD + metabolites. The new method incorporates new separations and improves upon a previously published method that suffered from the problem of ionization suppression for particular compounds.
Details
- Title: Subtitle
- Targeted, LCMS-based Metabolomics for Quantitative Measurement of NAD+ Metabolites
- Creators
- Samuel AJ Trammell - Interdisciplinary Graduate Program in Genetics Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USACharles Brenner - Interdisciplinary Graduate Program in Genetics Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Computational and Structural Biotechnology Journal, Vol.4(5), e201301012
- DOI
- 10.5936/csbj.201301012
- PMID
- 24688693
- PMCID
- PMC3962138
- NLM abbreviation
- Comput Struct Biotechnol J
- ISSN
- 2001-0370
- eISSN
- 2001-0370
- Publisher
- Research Network of Computational and Structural Biotechnology (RNCSB) Organization
- Grant note
- DOI: 10.13039/100000001, name: National Science Foundation and, award: MCB-0822581; name: NIH Pre-Doctoral Training Program in Genetics T32, award: GM008629
- Language
- English
- Date published
- 2013
- Academic Unit
- Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9983788431302771
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