Journal article
Targeting delivery oligonucleotide into macrophages by cationic polysaccharide from Bletilla striata successfully inhibited the expression of TNF-α
Journal of controlled release, Vol.134(3), pp.214-220
2009
DOI: 10.1016/j.jconrel.2008.11.013
PMID: 19073226
Abstract
Competent vehicles based on natural biopolymers are highly demanded in the practice of gene-assisted cell therapy. In this study, a novel gene carrier was developed based on a bioactive glucomannan that was a polysaccharide isolated from an herb
Bletilla striata (BSP) and modified with N, N′-carbonyldiimidazole (CDI)/ethylenediamine in order to acquire nucleic acid binding affinity. Particle size observation and electrophoretic mobility tests indicated that the cationized BSP (cBSP) could efficiently combine DNA to form nano-scaled compact and stable complexes and promote the transfection of oligodeoxynucleotide (ODN). Specifically, cBSP exhibited significantly high affinity to macrophages, as evidenced by transfection examination on multiple cell types and competitive test with mannose/glucomannan. In addition, the efficacy of the delivered ODN by cBSP was evaluated by the quantification of gene expression and a dramatic enhancement in suppressing target gene expression was observed. All the findings suggested the possible existence of interaction between cBSP ligand and receptor on macrophage surface. In this way, the ubiquitous mannose receptors and β-glucan receptors on macrophage could recognize the mannose and β-glucose residues in BSP framework, thus further mediated the oriented ODN delivery. We expect cBSP to be capable of conveying antisense nucleotides (e.g., oligodeoxynucleotide and small interference RNA) for the practical anti-inflammatory therapy.
Cationic polysaccharide from
Bletilla striata containing α-Mannose and β-Glucose monosaccharide contents exhibits high transfection effects and specificity to macrophage cell lines.
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Details
- Title: Subtitle
- Targeting delivery oligonucleotide into macrophages by cationic polysaccharide from Bletilla striata successfully inhibited the expression of TNF-α
- Creators
- Lei Dong - Nanjing UniversitySuhua Xia - Nanjing UniversityYi Luo - Nanjing UniversityHuajia Diao - Nanjing UniversityJiani Zhang - Nanjing UniversityJiangning Chen - Nanjing UniversityJunfeng Zhang - Nanjing University
- Resource Type
- Journal article
- Publication Details
- Journal of controlled release, Vol.134(3), pp.214-220
- Publisher
- Elsevier B.V
- DOI
- 10.1016/j.jconrel.2008.11.013
- PMID
- 19073226
- ISSN
- 0168-3659
- eISSN
- 1873-4995
- Language
- English
- Date published
- 2009
- Academic Unit
- Urology
- Record Identifier
- 9984320067702771
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