Journal article
Tau Binds to the Distal Axon Early in Development of Polarity in a Microtubule- and Microfilament-Dependent Manner
The Journal of neuroscience, Vol.16(18), pp.5583-5592
09/15/1996
DOI: 10.1523/JNEUROSCI.16-18-05583.1996
PMCID: PMC6578978
PMID: 8795614
Abstract
Microtubule-associated protein tau is localized to the axon
in situ
and has been implicated in the development of neuronal polarity. Here we report that tau is extracted differentially in cultured hippocampal neurons yielding an axon-specific localization under conditions that keep the integrity of the plasma membrane. The amount of bound tau increases toward the distal axon and is highest at the transition from the axonal shaft to the growth cone. This distribution is significantly different from the distribution of axonal microtubules that are most concentrated at the proximal axon. Distal binding of tau to one process appears early in development of polarity in culture and correlates with the onset of axon formation (day 2 in culture). Binding to the distal axon requires intact microtubules and microfilaments. Distal tau binding does not stabilize microtubules selectively against drug-induced disassembly, because colchicine-induced microtubule depolymerization is highest distally. We conclude that binding of tau to the distal axon follows a complex mechanism, is an early event in the development of polarity, and reproduces the axon-specific localization of tau
in situ
.
Details
- Title: Subtitle
- Tau Binds to the Distal Axon Early in Development of Polarity in a Microtubule- and Microfilament-Dependent Manner
- Creators
- Martina KempfAlbrecht ClementAndreas FaissnerGloria LeeRoland Brandt
- Resource Type
- Journal article
- Publication Details
- The Journal of neuroscience, Vol.16(18), pp.5583-5592
- Publisher
- Society for Neuroscience
- DOI
- 10.1523/JNEUROSCI.16-18-05583.1996
- PMID
- 8795614
- PMCID
- PMC6578978
- ISSN
- 0270-6474
- eISSN
- 1529-2401
- Language
- English
- Date published
- 09/15/1996
- Academic Unit
- Iowa Neuroscience Institute; Immunology; Internal Medicine
- Record Identifier
- 9984065393102771
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