Tau impacts on growth-factor-stimulated actin remodeling

Vandana M Sharma; Joel M Litersky; Kiran Bhaskar; Gloria Lee

doi:10.1242/jcs.03378

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Tau impacts on growth-factor-stimulated actin remodeling

Journal article

Open access

Peer reviewed

Tau impacts on growth-factor-stimulated actin remodeling

Vandana M Sharma, Joel M Litersky, Kiran Bhaskar and Gloria Lee

Journal of cell science, Vol.120(Pt 5), pp.748-757

03/01/2007

DOI: 10.1242/jcs.03378

PMID: 17284520

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Abstract

The microtubule-associated protein tau interacts with the SH3 domain of non-receptor Src family protein tyrosine kinases. A potential consequence of the SH3 interaction is the upregulation of tyrosine kinase activity. Here we investigated the activation of Src or Fyn by tau, both in vitro and in vivo. Tau increased the kinase activity in in vitro assays and in transfected COS7 cells. In platelet-derived growth factor (PDGF)-stimulated fibroblasts, tau appeared to prime Src for activation following PDGF stimulation, as reflected by changes in Src-mediated actin rearrangements. In addition, while fibroblasts normally recovered actin stress fibers by 5-7 hours after PDGF stimulation, tau-expressing cells showed sustained actin breakdown. Microtubule association by tau was not required for the observed changes in actin morphology. Inhibition of Src kinases or a mutant deficient in Src interaction reduced the effects, implicating Src family protein tyrosine kinases as a mediator of the effects of tau on actin rearrangements. Our results provide evidence that the interaction of tau with Src upregulates tyrosine kinase activity and that this interaction allows tau to impact on growth-factor-induced actin remodeling.

Humans

Actins - metabolism

Cercopithecus aethiops

tau Proteins - metabolism

Platelet-Derived Growth Factor - pharmacology

Gene Expression Regulation - drug effects

Tubulin - metabolism

Animals

Transfection

tau Proteins - genetics

tau Proteins - physiology

Fluorescent Antibody Technique

src-Family Kinases - metabolism

Cytoskeleton - metabolism

Mice

Phosphorylation - drug effects

COS Cells

3T3 Cells

Cytoskeleton - drug effects

Details

Title: Subtitle: Tau impacts on growth-factor-stimulated actin remodeling
Creators: Vandana M Sharma - Department of Internal Medicine, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
Joel M Litersky
Kiran Bhaskar
Gloria Lee
Resource Type: Journal article
Publication Details: Journal of cell science, Vol.120(Pt 5), pp.748-757
DOI: 10.1242/jcs.03378
PMID: 17284520
NLM abbreviation: J Cell Sci
ISSN: 0021-9533
eISSN: 1477-9137
Publisher: England
Language: English
Date published: 03/01/2007
Academic Unit: Iowa Neuroscience Institute; Immunology; Internal Medicine
Record Identifier: 9984065383202771

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