Journal article
Tau is required for neurite outgrowth and growth cone motility of chick sensory neurons
Cell motility and the cytoskeleton, Vol.43(3), pp.232-242
1999
DOI: 10.1002/(SICI)1097-0169(1999)43:3<232::AID-CM6>3.0.CO;2-7
PMID: 10401579
Abstract
The role of the microtubule‐associated protein (MAP) tau in axon growth remains controversial. Antisense experiments have suggested that tau is required for axon outgrowth, whereas genetic knockout and immunodepletion studies have suggested that tau plays no role in this process. To investigate the role of tau in both neurite outgrowth and growth cone motility, we have used a different approach, the chromophore‐assisted laser inactivation (CALI) of tau in chick dorsal root ganglion (DRG) neurons in culture. This approach generates an acute loss of tau function that is not subject to compensation by other MAPs. Inactivation of tau in whole DRG neurons (including cell body and neurites) reduced neurite number and length. Inactivation of tau within regions of growth cones using micro‐scale CALI caused a decrease in neurite extension rate by approximately 2‐fold. Surprisingly, it also caused a ∼20% decrease in the lamellipodial size within the inactivation region, whereas the filopodial motility was not affected. These results suggest that tau is required in neurite outgrowth and that tau also functions in lamellipodial motility at the growth cone leading edge. Cell Motil. Cytoskeleton 43:232–242, 1999. © 1999 Wiley‐Liss, Inc.
Details
- Title: Subtitle
- Tau is required for neurite outgrowth and growth cone motility of chick sensory neurons
- Creators
- Can‐wen A LiuGloria LeeDaniel G Jay
- Resource Type
- Journal article
- Publication Details
- Cell motility and the cytoskeleton, Vol.43(3), pp.232-242
- Publisher
- John Wiley & Sons, Inc; New York
- DOI
- 10.1002/(SICI)1097-0169(1999)43:3<232::AID-CM6>3.0.CO;2-7
- PMID
- 10401579
- ISSN
- 0886-1544
- eISSN
- 1097-0169
- Number of pages
- 11
- Grant note
- Program in Mathematics and Molecular Biology Klingenstein Fellowship Award in the Neurosciences National Institute of Health (NIH) (NS‐34699; NS32100) National Science Foundation (DMS‐9406348)
- Language
- English
- Date published
- 1999
- Academic Unit
- Iowa Neuroscience Institute; Immunology; Internal Medicine
- Record Identifier
- 9984065483402771
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