Journal article
Tau protein binds single-stranded DNA sequence specifically – the proof obtained in vitro with non-equilibrium capillary electrophoresis of equilibrium mixtures
FEBS letters, Vol.579(6), pp.1371-1375
2005
DOI: 10.1016/j.febslet.2005.01.032
PMID: 15733843
Abstract
Tau is a microtubule-associated protein, which plays an important role in physiology and pathology of neurons. Tau has been recently reported to bind double-stranded DNA (dsDNA) but not to bind single-stranded DNA (ssDNA) [Cell. Mol. Life Sci. 2003, 60, 413–421]. Here, we prove that tau binds not only dsDNA but also ssDNA. This finding was facilitated by using two kinetic capillary electrophoresis methods: (i) non-equilibrium capillary electrophoresis of equilibrium mixtures (NECEEM); (ii) affinity-mediated NECEEM. Using the new approach, we observed, for the first time, that tau could induce dissociation of strands in dsDNA by binding one of them in a sequence-specific fashion. Moreover, we determined the equilibrium dissociation constants for all tau–DNA complexes studied.
Details
- Title: Subtitle
- Tau protein binds single-stranded DNA sequence specifically – the proof obtained in vitro with non-equilibrium capillary electrophoresis of equilibrium mixtures
- Creators
- Svetlana M Krylova - Department of Chemistry, York University, Toronto, Ont., Canada M3J 1P3Michael Musheev - Department of Chemistry, York University, Toronto, Ont., Canada M3J 1P3Razvan Nutiu - Departments of Biochemistry and Chemistry, McMaster University, Hamilton, Ont., Canada L8N 3Z5Yingfu Li - Departments of Biochemistry and Chemistry, McMaster University, Hamilton, Ont., Canada L8N 3Z5Gloria Lee - Departments of Internal Medicine, University of Iowa, 200 Hawkins Drive, Iowa City, Iowa 52242, USASergey N Krylov - Department of Chemistry, York University, Toronto, Ont., Canada M3J 1P3
- Resource Type
- Journal article
- Publication Details
- FEBS letters, Vol.579(6), pp.1371-1375
- Publisher
- Elsevier B.V
- DOI
- 10.1016/j.febslet.2005.01.032
- PMID
- 15733843
- ISSN
- 0014-5793
- eISSN
- 1873-3468
- Language
- English
- Date published
- 2005
- Academic Unit
- Iowa Neuroscience Institute; Immunology; Internal Medicine
- Record Identifier
- 9984065388602771
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