Journal article
Telomerase activation in cervical cancer
The American journal of pathology, Vol.151(1), pp.25-31
07/1997
PMCID: PMC1857907
PMID: 9212727
Abstract
It has been hypothesized that infection with high-risk human papillomaviruses (HPVs), in conjunction with other cellular events, plays a critical role in the development of cervical cancer. Activation of telomerase, a ribonucleoprotein enzyme complex that synthesizes telomere repeats, has been associated with acquisition of the immortal phenotype in vitro and is commonly observed in human cancers. In this study, we have examined 10 high-grade cervical cancers for telomerase activity and for the presence of HPV. Telomerase activity was detected in all of the cancers but in none of the paired histopathologically normal uterine tissues or in normal cervical epithelium. Analysis of these same tissues for HPV nucleic acids by polymerase chain reaction (PCR) using primers from the HPV L1 and E6 open reading frames demonstrated that 7 of 10 cancers were positive for HPV, 3 for HPV type 16 (HPV-16), and 4 for HPV-18. In one case, HPV-16 was detected in histopathologically normal uterine tissue, the same type as that detected in the cancer from the same patient. HPV DNA was not detected in 3 of 10 cancers. These results indicate that telomerase activation is common in high-grade cervical cancers and suggests that telomerase activity may be a useful diagnostic marker for the disease.
Details
- Title: Subtitle
- Telomerase activation in cervical cancer
- Creators
- Suzanne Anderson - Program in Cancer Biology, Fred Hutchinson Cancer Research Center, University of Washington, Seattle 98104, USAKatherine Shera - Program in Cancer Biology, Fred Hutchinson Cancer Research Center, University of Washington, Seattle 98104, USAJennifer Ihle - Program in Cancer Biology, Fred Hutchinson Cancer Research Center, University of Washington, Seattle 98104, USALisa Billman - Program in Cancer Biology, Fred Hutchinson Cancer Research Center, University of Washington, Seattle 98104, USABarbara Goff - Program in Cancer Biology, Fred Hutchinson Cancer Research Center, University of Washington, Seattle 98104, USABenjamin Greer - Program in Cancer Biology, Fred Hutchinson Cancer Research Center, University of Washington, Seattle 98104, USAHisham Tamimi - Program in Cancer Biology, Fred Hutchinson Cancer Research Center, University of Washington, Seattle 98104, USAJames McDougall - Program in Cancer Biology, Fred Hutchinson Cancer Research Center, University of Washington, Seattle 98104, USAA Klingelhutz - Program in Cancer Biology, Fred Hutchinson Cancer Research Center, University of Washington, Seattle 98104, USA
- Resource Type
- Journal article
- Publication Details
- The American journal of pathology, Vol.151(1), pp.25-31
- PMID
- 9212727
- PMCID
- PMC1857907
- ISSN
- 0002-9440
- eISSN
- 1525-2191
- Language
- English
- Date published
- 07/1997
- Academic Unit
- Microbiology and Immunology; Radiation Oncology
- Record Identifier
- 9984001125202771
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