Journal article
Temperature-Dependent Developmental Plasticity of Drosophila Neurons: Cell-Autonomous Roles of Membrane Excitability, Ca2+ Influx, and cAMP Signaling
The Journal of neuroscience, Vol.27(46), pp.12611-12622
11/14/2007
DOI: 10.1523/JNEUROSCI.2179-07.2007
PMID: 18003840
Abstract
Environmental temperature is an important factor exerting pervasive influence on neuronal morphology and synaptic physiology. In the
Drosophila
brain, axonal arborization of mushroom body Kenyon cells was enhanced when flies were raised at high temperature (30°C rather than 22°C) for several days. Isolated embryonic neurons in culture that lacked cell–cell contacts also displayed a robust temperature-induced neurite outgrowth. This cell-autonomous effect was reflected by significantly increased high-order branching and enlarged growth cones. The temperature-induced morphological alterations were blocked by the Na
+
channel blocker tetrodotoxin and a Ca
2+
channel mutation but could be mimicked by raising cultures at room temperature with suppressed K
+
channel activity. Physiological analyses revealed increased inward Ca
2+
currents and decreased outward K
+
currents, in conjunction with a distal shift in the site of action potential initiation and increased prevalence of TTX-sensitive spontaneous Ca
2+
transients. Importantly, the overgrowth caused by both temperature and hyperexcitability K
+
channel mutations were sensitive to genetic perturbations of cAMP metabolism. Thus, temperature acts in a cell-autonomous manner to regulate neuronal excitability and spontaneous activity. Presumably, activity-dependent Ca
2+
accumulation triggers the cAMP cascade to confer the activity-dependent plasticity of neuronal excitability and growth.
Details
- Title: Subtitle
- Temperature-Dependent Developmental Plasticity of Drosophila Neurons: Cell-Autonomous Roles of Membrane Excitability, Ca2+ Influx, and cAMP Signaling
- Creators
- I-Feng PengBrett A BerkeYue ZhuWei-Hua LeeWenjia ChenChun-Fang Wu
- Resource Type
- Journal article
- Publication Details
- The Journal of neuroscience, Vol.27(46), pp.12611-12622
- Publisher
- Society for Neuroscience
- DOI
- 10.1523/JNEUROSCI.2179-07.2007
- PMID
- 18003840
- ISSN
- 0270-6474
- eISSN
- 1529-2401
- Language
- English
- Date published
- 11/14/2007
- Academic Unit
- Iowa Neuroscience Institute; Biology
- Record Identifier
- 9984070205302771
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