Journal article
Temperature-Insensitive Near-Infrared Spectroscopic Measurement of Glucose in Aqueous Solutions
Applied spectroscopy, Vol.48(4), pp.477-483
04/1994
DOI: 10.1366/000370294775268910
Abstract
A digital Fourier filter is combined with partial least-squares (PLS) regression to generate a calibration model for glucose that is insensitive to sample temperature. This model is initially created by using spectra collected over the 5000 to 4000 cm−1 spectral range with samples maintained at 37°C. The analytical utility of the model is evaluated by judging the ability to determine glucose concentrations from a set of prediction spectra. Absorption spectra in this prediction set are obtained by ratioing single-beam spectra collected from solutions at temperatures ranging from 32 to 41°C to reference spectra collected at 37°C. The temperature sensitivity of the underlying water absorption bands creates large baseline variations in prediction spectra that are effectively eliminated by the Fourier filtering step. The best model provides a mean standard error of prediction across temperatures of 0.14 mM (2.52 mg/dL). The benefits of the Fourier filtering step are established, and critical experimental parameters, such as number of PLS factors, mean and standard deviation for the Gaussian shaped Fourier filter, and spectral range, are considered.
Details
- Title: Subtitle
- Temperature-Insensitive Near-Infrared Spectroscopic Measurement of Glucose in Aqueous Solutions
- Creators
- Kevin H Hazen - Department of Chemistry, Iowa City, Iowa 52242 (K.H.H., M.A.A.); and Center for Intelligent Chemical Instrumentation, Department of Chemistry, Clippinger Laboratories, Ohio University, Athens, Ohio 45701-2979 (G. W.S.)Mark A Arnold - Department of Chemistry, Iowa City, Iowa 52242 (K.H.H., M.A.A.); and Center for Intelligent Chemical Instrumentation, Department of Chemistry, Clippinger Laboratories, Ohio University, Athens, Ohio 45701-2979 (G. W.S.)Gary W Small - Department of Chemistry, Iowa City, Iowa 52242 (K.H.H., M.A.A.); and Center for Intelligent Chemical Instrumentation, Department of Chemistry, Clippinger Laboratories, Ohio University, Athens, Ohio 45701-2979 (G. W.S.)
- Resource Type
- Journal article
- Publication Details
- Applied spectroscopy, Vol.48(4), pp.477-483
- Publisher
- SAGE Publications
- DOI
- 10.1366/000370294775268910
- ISSN
- 0003-7028
- eISSN
- 1943-3530
- Language
- English
- Date published
- 04/1994
- Academic Unit
- Fraternal Order of Eagles Diabetes Research Center; Center for Biocatalysis and Bioprocessing; Chemistry
- Record Identifier
- 9984216564102771
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