Temperature change does not affect force between single actin filaments and HMM from rabbit muscles

M Kawai; K Kawaguchi; M Saito; S Ishiwata

doi:10.1016/S0006-3495(00)76848-2

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Temperature change does not affect force between single actin filaments and HMM from rabbit muscles

Journal article

Open access

Peer reviewed

Temperature change does not affect force between single actin filaments and HMM from rabbit muscles

M Kawai, K Kawaguchi, M Saito and S Ishiwata

Biophysical journal, Vol.78(6), pp.3112-3119

06/2000

DOI: 10.1016/S0006-3495(00)76848-2

PMCID: PMC1300893

PMID: 10827988

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Abstract

The temperature dependence of sliding force, velocity, and unbinding force was studied on actin filaments when they were placed on heavy meromyosin (HMM) attached to a glass surface. A fluorescently labeled actin filament was attached to the gelsolin-coated surface of a 1-microm polystyrene bead. The bead was trapped by optical tweezers, and HMM-actin interaction was performed at 20-35 degrees C to examine whether force is altered by the temperature change. Our experiments demonstrate that sliding force increased moderately with temperature (Q(10) = 1.6 +/- 0.2, +/-SEM, n = 9), whereas the velocity increased significantly (Q(10) = 2.9 +/- 0.4, n = 10). The moderate increase in force is caused by the increased number of available cross-bridges for actin interaction, because the cross-bridge number similarly increased with temperature (Q(10) = 1. 5 +/- 0.2, n = 3) when measured during rigor induction. We further found that unbinding force measured during the rigor condition did not differ with temperature. These results indicate that the amount of force each cross-bridge generates is fixed, and it does not change with temperature. We found that the above generalization was not modified in the presence of 1 mM MgADP or 8 mM phosphate.

Actins - chemistry

Actins - metabolism

Animals

Gelsolin

Kinetics

Muscle, Skeletal - physiology

Myosin Subfragments - chemistry

Myosin Subfragments - metabolism

Polystyrenes

Rabbits

Stress, Mechanical

Thermodynamics

Details

Title: Subtitle: Temperature change does not affect force between single actin filaments and HMM from rabbit muscles
Creators: M Kawai - University of Iowa
K Kawaguchi - Waseda University
M Saito - Waseda University
S Ishiwata - Waseda University
Resource Type: Journal article
Publication Details: Biophysical journal, Vol.78(6), pp.3112-3119
DOI: 10.1016/S0006-3495(00)76848-2
PMID: 10827988
PMCID: PMC1300893
NLM abbreviation: Biophys J
ISSN: 0006-3495
eISSN: 1542-0086
Language: English
Date published: 06/2000
Academic Unit: Anatomy and Cell Biology; Internal Medicine
Record Identifier: 9984284354202771

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